Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-PDGF Receptor β (Tyr1009) (42F9) Rabbit mAb #3124

No. Size Price
3124S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3124T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
3124 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 190 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-PDGF Receptor β (Tyr1009) (42F9) Rabbit mAb detects endogenous levels of PDGF receptor β only when phosphorylated at Tyr1009. The antibody may slightly cross-react with other activated PDGF receptor family members and other activated protein tyrosine kinases.

磷酸化PDGF Receptor β (Tyr1009) (42F9) 兔单抗仅在Tyr1009被磷酸化后才能检测到PDGF receptor β的存在。本抗体也能与其它激活的PDGF受体家族的成员或其它激活的酪氨酸蛋白激酶发生轻微的交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1009 of human PDGF receptor β.

单克隆抗体通过用多肽免疫动物得到,该磷酸化多肽是根据人的PDGF receptor β蛋白Tyr1009附近的氨基酸序列合成的。

Western Blotting

Western Blotting

Western blot analysis of cell extracts from NIH/3T3 cells unstimulated or stimulated with PDGF-BB (100 ng/ml for 5 min), using Phospho-PDGF Receptor-β (Tyr1009) (42F9) Rabbit mAb (upper) or PDGF receptor-β (2B3) Mouse mAb #3175 (lower).用Phospho-PDGF Receptor-β (Tyr1009) (42F9) Rabbit mAb (上) 或PDGF receptor-β (2B3) Mouse mAb #3175 (下) 对以下细胞的提取物进行免疫印迹检测:未处理的或PDGF-BB (100 ng/ml , 5 min)处理的NIH/3T3 细胞。


The proteins of the platelet derived growth factor (PDGF) family exist as several disulphide-bonded, dimeric isoforms (PDGF AA, PDGF AB, PDGF BB, PDGF CC, and PDGF DD) that bind in a specific pattern to two closely related receptor tyrosine kinases, PDGF receptor α (PDGFRα) and PDGF receptor β (PDGFRβ). PDGFRα and PDGFRβ share 75% to 85% sequence homology between their two intracellular kinase domains while the kinase insert and carboxy-terminal tail regions display a lower level (27% to 28%) of homology (1). PDGFRα homodimers bind all PDGF isoforms except those containing PDGF D. PDGFRβ homodimers bind PDGF BB and DD isoforms, as well as the PDGF AB heterodimer. The heteromeric PDGF receptor α/β binds PDGF B, C, and D homodimers as well as the PDGF AB heterodimer (2). PDGFRα and PDGFRβ can each form heterodimers with EGFR, which is also activated by PDGF (3). Various cells differ in the total number of receptors present and in the receptor subunit composition, which may account for responsive differences among cell types to PDGF binding (4). Ligand binding induces receptor dimerization and autophosphorylation, followed by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules such as Grb2, Src, GAP, PI3 kinase, PLCγ, and Nck. A number of different signaling pathways are initiated by activated PDGF receptors and lead to control of cell growth, actin reorganization, migration, and differentiation (5). Tyr751 in the kinase-insert region of PDGFRβ is the docking site for PI3 kinase (6). Phosphorylated pentapeptides derived from Tyr751 of PDGFRβ (pTyr751-Val-Pro-Met-Leu) inhibit the association of the carboxy-terminal SH2 domain of the p85 subunit of PI3 kinase with PDGFRβ (7). Tyr740 is also required for PDGFRβ-mediated PI3 kinase activation (8).

血小板衍生生长因子(PDGF) 家族的蛋白以几种二硫键连接的二聚体形式存在 (PDGF AA, PDGF AB, PDGF BB, PDGF CC和PDGF DD),它们以特定的方式与两种密切相关的酪氨酸激酶受体-PDGF receptor α (PDGFRα)和PDGF receptor β (PDGFRβ)-结合。PDGFRα 和PDGFRβ 的胞内激酶区有75% 到85% 的序列同源性,而激酶插入区和羧基端仅有很低的同源性(27% 到 28%) (1). PDGFRα 同型二聚体和除了含有PDGF D 之外的所有的PDGF亚型结合。PDGFRβ同型二聚体能结合PDGF BB 和DD亚型,还有PDGF AB 异二聚体。 异聚体 PDGF receptor α/β能结合 PDGF B, C, 和 D 同型二聚体,还有 PDGF AB 异二聚体 (2)。 PDGFRα 和 PDGFRβ 都能被PDGF激活和 EGFR形成异二聚体, (3)。各种细胞的受体总数、受体亚基组成都不相同,因此不同细胞对PDGF的反应也不同(4)。配体结合能诱发受体的二聚化和自磷酸化,继而结合并激活胞内含有SH2结构的信号传导分子, 如Grb2, Src, GAP, PI3K激酶, PLCγ和 Nck。激活的PDGFRs能引发一系列不同的信号通路 并影响细胞的生长、肌动蛋白重塑、迁移和分化 (5)。PDGFRβ激酶插入区的Tyr751位点结合PI3K激酶 (6)。根据PDGFRβ的Tyr751位点附近序列产生的磷酸化五肽(pTyr751-Val-Pro-Met-Leu)能够抑制PI3K激酶p85亚基的羧基端SH2区和PDGFRβ结合 (7)。 Tyr740对 PDGFRβ介导的 PI3 K激酶的激活也是必需的(8)。

Activation of the PDGFRβ leads to autophosphorylation on a number of tyrosine residues, including Tyr1009. Mutation analysis has shown that PDGF-stimulated PLCγ signaling is dependent on autophosphorylation of the PDGFRβ at Tyr1009 and Tyr1021 (9). Phosphorylated Tyr1009 also serves as a binding site for SHP-2, a SH2 domain-containing tyrosine phosphatase that is tyrosine-phosphorylated by PDGFRβ (10).

PDGFRβ的激活会导致包含Tyr1009在内的一系列酪氨酸位点的自磷酸化。突变分析显示PDGF激活的PLCγ 信号通路依赖于PDGFRβ的Tyr1009 和Tyr1021自磷酸化 (9)。 磷酸化的Tyr1009 又能为SHP-2提供结合位点,SHP-2是一个酪氨酸被PDGFRβ磷酸化的 SH2结构 (10)。

  1. Deuel, T.F. et al. (1988) Biofactors 1, 213-217.
  2. Bergsten, E. et al. (2001) Nat. Cell Biol. 3, 512-516.
  3. Betsholtz, C. et al. (2001) Bioessays 23, 494-507.
  4. Coughlin, S.R. et al. (1988) Prog. Clin. Biol. Res. 266, 39-45.
  5. Ostman, A. and Heldin, C.H. (2001) Adv. Cancer Res. 80, 1-38.
  6. Panayotou, G. et al. (1992) EMBO J. 11, 4261-4272.
  7. Ramalingam, K. et al. (1995) Bioorg. Med. Chem. 3, 1263-1272.
  8. Kashishian, A. et al. (1992) EMBO J. 11, 1373-1382.
  9. Rönnstrand, L. et al. (1992) EMBO J. 11, 3911-3919.
  10. Rönnstrand, L. et al. (1999) Oncogene 18, 3696-3702.

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