Cell Signaling Technology

Product Pathways - NF-kB Signaling

IL-1β (D6D6T) Rabbit mAb (Mouse Specific) #31202

No. Size Price
31202S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
31202 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse, Endogenous 17, 31 Rabbit IgG
F 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, F=Flow Cytometry,

Specificity / Sensitivity

IL-1β (D6D6T) Rabbit mAb (Mouse Specific) recognizes endogenous levels of total IL-1β protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to mouse IL-1β protein.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Raw264.7 cells, untreated (blue) or treated with LPS (100 ng/ml, 6 hr) (green) using IL-1β (D6L6T) Rabbit mAb (Mouse Specific). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from Raw264.7 cells, untreated (-) or treated with LPS (100 ng/mL, 6 hr; +), using IL-1β (D6D6T) Rabbit mAb (Mouse Specific) (upper), or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of recombinant mouse IL-1β (mIL-1β) using IL-1β (D6D6T) Rabbit mAb (Mouse Specific).

Background

Interleukin-1β (IL-1β), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses (1). It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets (2-6). Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form (7,8). Detection of the 17 kDa mature form of IL-1β is a good indicator of caspase-1 activity.

  1. Dinarello, C.A. (1998) Int Rev Immunol 16, 457-99.
  2. Burns, K. et al. (1998) J Biol Chem 273, 12203-9.
  3. Cao, Z. et al. (1996) Nature 383, 443-6.
  4. Cao, Z. et al. (1996) Science 271, 1128-31.
  5. Wesche, H. et al. (1997) Immunity 7, 837-47.
  6. Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-6.
  7. Thornberry, N.A. et al. (1992) Nature 356, 768-74.
  8. Cerretti, D.P. et al. (1992) Science 256, 97-100.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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