Cell Signaling Technology

Product Pathways - Metabolism

HNF4α (C11F12) Rabbit mAb #3113

Hepatocyte nuclear factor   Hepatocyte nuclear factor 4   Hepatocyte nuclear factor 4-alpha   HNF   HNF4   HNF4a   HNF4alpha   NR2A1   sc-6556   sc-8987   TCF14   Transcription factor 14  

No. Size Price
3113S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
3113 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 52 Rabbit IgG
IHC-P 1:200
IF-IC 1:6000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

HNF4α (C11F12) Rabbit mAb detects endogenous levels of total HNF4α protein.

HNF4α (C11F12)兔单抗抗体识别内源性的HNF4α总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human HNF4α.

该单克隆抗体用与人类HNF4α蛋白氨基酸序列对应的人工合成肽段免疫动物制成。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HepG2 (left) and HeLa cells (right) using HNF4α (C11F12) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).对HepG2细胞(左)和HeLa细胞(右)使用HNF4α (C11F12) Rabbit mAb(绿色)进行共聚焦免疫荧光分析。肌动蛋白用Alexa Fluor? 555 phalloidin(红色)标记。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HepG2 cells in the presence of control peptide (left) or antigen specific peptide (right) using HNF4α (C11F12E8) Rabbit mAb.对石蜡包埋的HepG2细胞使用HNF4α (C11F12) Rabbit mAb抗体进行免疫组织化学分析,其中左图为对照肽,右图为抗原特异肽。

Western Blotting

Western Blotting

Western blot analysis of extract from HepG2 cells using HNF4α (C11F12) Rabbit mAb.对HepG2细胞抽提液使用HNF4α (C11F12) Rabbit mAb进行Western blot分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin embedded human hepatocellular carcinoma using HNF4α (C11F12) Rabbit mAb.对石蜡包埋的人肝细胞癌使用HNF4α(C11F12) Rabbit mAb进行免疫组化分析。

Background

Hepatocyte nuclear factor 4α (HNF4α) is a transcription factor that belongs to the steroid hormone receptor superfamily and is enriched in liver (1). HNF4α, in association with PGC-1α, activates gluconeogenic genes such as phosphoenolpyruvate carboxykinase and glucose-6-phosphatase genes in fasted livers (2,3). Conditional knockout of the HNF4α gene in the mouse liver destroys lipid homeostasis and leads to lipid accumulation in the liver and a reduction of serum cholesterol and triglyceride levels (4). Mutations in HNF4α have been linked to maturity-onset diabetes of the young (MODY) (5).

肝细胞核因子4α(HNF4α)是一个转录因子,属于类固醇激素受体超家族,在肝脏中富含(1)。HNF4α与PGC-1α协同,刺激空腹时肝脏生糖基因如磷酸烯醇式丙酮酸羧激酶和葡萄糖-6-磷酸酶的激活(2,3)。条件下敲除小鼠肝脏HNF4α基因会破坏脂质平衡,导致脂肪在肝脏堆积,以及血浆胆固醇和甘油三酯水平的降低(4)。HNF4α突变与成年发病的早发型糖尿病(MODY)相关(5)。

  1. Sladek, F.M. et al. (1990) Genes Dev. 4, 2353-2365.
  2. Yoon, J.C. et al. (2001) Nature 413, 131-138.
  3. Rhee, J. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 4012-4017.
  4. Hayhurst, G.P. et al. (2001) Mol. Cell Biol. 21, 1393-1403.
  5. Ellard, S. and Colclough, K. (2006) Hum. Mutat. 27, 854-869.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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