Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-VASP (Ser157) Antibody #3111

VASP  

No. Size Price
3111S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3111T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
3111 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,Guinea Pig, Endogenous 50 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-VASP (Ser157) Antibody detects endogenous levels of VASP only when phosphorylated at serine 157. The antibody may cross-react with the phosphorylated VASP homologue Mena.

Phospho-VASP (Ser157) Antibody检测仅在serine 157位点磷酸化的内源性VASP蛋白。该抗体可能与磷酸化的VASP同系物例如Mena蛋白发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser157 of human VASP. Antibodies are purified by protein A and peptide affinity chromatography.

通过人工合成人源VASP蛋白Ser157位点周围相应的磷酸化片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 and C6 cells, untreated, 8-Br-cGMP-treated, 8-Br-cAMP-treated or forskolin-treated as indicated, using Phospho-VASP (Ser157) Antibody.

使用Phospho-VASP (Ser157) Antibody,免疫印迹(Western blot)分析NIH/3T3和C6细胞,细胞分别经untreated、8-Br-cGMP-treated、8-Br-cAMP-treated或forskolin-treated如图所示。

Western Blotting

Western Blotting

Western blot analysis of extracts from A-431 cells, untreated (-) or treated with Forskolin #3828 (+), using Phospho-VASP (Ser157) Antibody (upper) and VASP (9A2) Rabbit mAb #3132 (lower).

Background

Vasodilator-stimulated phosphoprotein (VASP) was originally characterized as a substrate of both cGMP- and cAMP-dependent kinases (PKG and PKA, or cGPK and cAPK, respectively) (1). It is now believed that VASP belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways and that it functions in cytoskeletal organization, fibroblast migration, platelet activation and axon guidance (2,3). Three phosphorylation sites, Ser157, Ser239, and Thr278, have been identified. Ser239 is the major PKG phosphorylation site while Ser157 is the major PKA phosphorylation site (4). Evidence suggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization (5). Phosphorylation at Ser239 of VASP is a useful marker for monitoring PKG activation and signaling (6,7).

Vasodilator-stimulated phosphoprotein (VASP)起初的特征是分别作为cGMP- 和cAMP-依赖的激酶(PKG和PKA或cGPK 和cAPK)的一个底物(1)。研究认为VASP蛋白属于受体蛋白的Ena/VASP家族,而受体蛋白是可将细胞骨架系统联系到信号转导通路,并且其在细胞骨架的组织、成纤维细胞迁移、血小板活化和轴突导向中有一定作用(2,3)。三个磷酸化位点Ser157、Ser239和Thr278已经被鉴定。Ser239位点是主要的PKG磷酸化位点,而Ser157位点是主要的PKA磷酸化位点(4)。研究证明VASP磷酸化可减少它与actin的联系,并且有一个负性影响在actin的聚合作用 (5)。VASP蛋白Ser239位点的磷酸化是对监控PKG激活和信号发送起到一个很好的标记物的作用(6,7)。

  1. Butt, E. et al. (1994) J Biol Chem 269, 14509-17.
  2. Ball, L.J. et al. (2000) EMBO J 19, 4903-14.
  3. Machesky, L.M. (2000) Cell 101, 685-8.
  4. Smolenski, A. et al. (1998) J Biol Chem 273, 20029-35.
  5. Harbeck, B. et al. (2000) J Biol Chem 275, 30817-25.
  6. Oelze, M. et al. (2000) Circ Res 87, 999-1005.
  7. Lawrence, D.W. and Pryzwansky, K.B. (2001) J Immunol 166, 5550-6.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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