Cell Signaling Technology

Product Pathways - TGF-beta/Smad Signaling

Phospho-Smad2 (Ser245/250/255) Antibody #3104

smad   smad2  

No. Size Price
3104S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
3104 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 60 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Rat,

Specificity / Sensitivity

Phospho-Smad2 (Ser245/250/255) Antibody detects endogenous levels of Smad2 only when phosphorylated at serines 245, 250 or 255.

磷酸化的Smad2(Ser 245/250/255) 抗体只识别在丝氨酸245, 250 或255处磷酸化的内源性Smad2蛋白.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding serines 245/250/255 of Smad2. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from 293 and HeLa cells, untreated or TPA-treated (200 nM for 30 minutes) using Phospho-Smad2 (Ser245/250/255) Antibody (upper) or Smad2 Antibody (lower).

使用Phospho-Smad2 (Ser245/250/255) 抗体(上)或Smad2抗体(下)对未处理或经过TPA处理(200 nM,30分钟)的293和HeLa细胞提取物进行western blot分析。


Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8).

Smad信号转导分子家族成员是胞内通路中TGF-β信号从细胞表面传送到细胞核的重要组成部分。Smad 可被分为三类:受体调控型Smad(简称R-Smad),主要包括Smad1,2,3,5和8;共同介导型Smad(co-Smad)包括Smad4;还有拮抗型或称抑制型Smad(I-Smads),包括Smad6和Smad7[1-5]。激活的I型受体与特异的R-Smad有关,并能在R-Smad的保守的C端SSXS基序处对其磷酸化。磷酸化的R-Smad与受体分离,再与co-Smad(Smad4)形成异侧复合物,使复合物迁移入核。一旦进入核内,Smad可以各种DNA结合蛋白为目标调控转录反应。[6-8]

Oncogenic Ras antagonizes TGF-beta signaling and inhibits the nuclear accumulation of Smad2 and Smad3, which may be explained through MAP kinase dependent phosphorylation of these Smads (9).Cell stimulation with EGF leads to phosphorylation of Smad2 at a cluster of serine-proline sites within its linker region, including Ser245, 250, and 255 (9).

癌基因Ras阻碍的TGF-beta信号通路并且抑制Smad2和3在核内累积,这也可能由MAP 激酶依赖的Smad磷酸化参与调控(9)。EGF刺激的细胞会导致Smad2在一簇丝氨酸-脯氨酸位点内部的连接区域的磷酸化,包括丝氨酸位点245,250和255(9).

  1. Heldin, C.H. et al. (1997) Nature 390, 465-71.
  2. Attisano, L. and Wrana, J.L. (1998) Curr Opin Cell Biol 10, 188-94.
  3. Derynck, R. et al. (1998) Cell 95, 737-40.
  4. Massagué, J. (1998) Annu Rev Biochem 67, 753-91.
  5. Whitman, M. (1998) Genes Dev 12, 2445-62.
  6. Wu, G. et al. (2000) Science 287, 92-7.
  7. Attisano, L. and Wrana, J.L. (2002) Science 296, 1646-7.
  8. Moustakas, A. et al. (2001) J Cell Sci 114, 4359-69.
  9. Kretzschmar, M. et al. (1999) Genes Dev 13, 804-16.

Application References

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