Cell Signaling Technology

Product Pathways - Nuclear Receptor Signaling

RXRα (D6H10) Rabbit mAb #3085


No. Size Price
3085S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
3085 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 53 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

RXRα (D6H10) Rabbit mAb recognizes endogenous levels of total RXRα protein. This antibody does not cross-react with either RXRβ or RXRγ.RXRα (D6H10) Rabbit mAb识别内源性水平的总RXRα蛋白。该抗体不与RXRβ或RXRγ中的任何一个发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RXRα protein.该单克隆抗体通过用合成肽免疫动物制备,该合成肽是人RXRα蛋白靠近氨基末端的残基。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using RXRα (D6H10) Rabbit mAb.Western blot方法检测多个细胞系提取物,使用的抗体为RXRα (D6H10) Rabbit mAb.

Isoform Specificity

Isoform Specificity

Western blot analysis of extracts from 293T cells, either mock transfected (-) or transfected with Myc/DDK-tagged cDNA expression constructs encoding full-length human RXRα (hRXRα; +), RXRβ (hRXRβ; +), or RXRγ (hRXRγ; +), using RXRα (D6H10) Rabbit mAb (upper) and DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #2368 (lower).Western blot方法检测293T细胞提取物,细胞不转染或用Myc/DDK标记的cDNA表达构建体转染,该构建体编码人全长RXRα (hRXRα; +), RXRβ (hRXRβ; +)或RXRγ (hRXRγ; +),使用的抗体为RXRα (D6H10) Rabbit mAb(上图)或DYKDDDDK Tag Antibody (与Sigma的Anti-FLAG® M2 Antibody结合相同的抗原表位) #2368 (下图)。


The human retinoid X receptors (RXRs) are encoded by three distinct genes (RXRα, RXRβ, and RXRγ) and bind selectively and with high affinity to the vitamin A derivative, 9-cis-retinoic acid. RXRs are type-II nuclear hormone receptors that are largely localized to the nuclear compartment independent of ligand binding. Nuclear RXRs form heterodimers with nuclear hormone receptor subfamily 1 proteins, including thyroid hormone receptor, retinoic acid receptors, vitamin D receptor, peroxisome proliferator-activated receptors, liver X receptors, and farnesoid X receptor (1). Since RXRs heterodimerize with multiple nuclear hormone receptors, they play a central role in transcriptional control of numerous hormonal signaling pathways by binding to cis-acting response elements in the promoter/enhancer region of target genes (2).人视黄醇X受体(RXRs)由三种不同的基因(RXRα,RXRβ,RXRγ)编码,并与具有高亲和力的维生素A衍生物,9-顺-视黄酸选择性结合。 RXRs是II型核激素受体,主要位于核室,非依赖性配体结合。核RXR与核激素受体亚家族蛋白形成异二聚体,包括甲状腺激素受体、维甲酸受体、维生素D受体、过氧化物酶体增殖物激活受体、肝脏X受体、法尼酯X受体(1)。由于RXR与多个核激素受体形成异源二聚体,通过与靶基因启动子/增强区域的顺式作用元件结合,在众多激素信号转导通路的转录调控中发挥核心作用(2)。

Retinoid X receptor α (RXRα) is the founding RXR family member and is predominantly expressed in the liver, kidney, epidermis, intestine, and a variety of tissues (2-4). Knockout of the murine rxrα gene results in embryonic lethality tentatively due to myocardial hypoplasia, which demonstrates the importance of RXRα in retinoid signaling in vivo (5,6). Biochemical evidence suggests that RXRα transcriptional activity is post-translationally regulated through the Ras-Raf-MAPK signaling cascade. MAPK-dependent phosphorylation of RXRα directly abrogates the ability of RXRα to associate with nuclear receptor coactivators (7).维甲酸X受体α(RXRα)是创始RXR受体家族成员,主要表达在肝、肾、表皮、肠和各种组织中(2-4)。基因敲除小鼠RXRα基因会导致胚胎心肌发育不全使胚胎致死,这也证明 RXRα对体内视黄醇信号的重要性(5,6)。生化证据表明,RXRα转录活性是通过 Ras-Raf-MAPK信号级联在翻译后调节的。MAPK依赖性RXRα的磷酸化直接去除RXRα与核受体共激活因子关联的能力(7)。

  1. Gronemeyer, H. et al. (2004) Nat Rev Drug Discov 3, 950-64.
  2. Mangelsdorf, D.J. et al. (1992) Genes Dev 6, 329-44.
  3. Mangelsdorf, D.J. et al. (1990) Nature 345, 224-9.
  4. Dollé, P. et al. (1994) Mech Dev 45, 91-104.
  5. Kastner, P. et al. (1994) Cell 78, 987-1003.
  6. Sucov, H.M. et al. (1994) Genes Dev 8, 1007-18.
  7. Macoritto, M. et al. (2008) J Biol Chem 283, 4943-56.

Application References

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