Cell Signaling Technology

Product Pathways - Metabolism

Phospho-C/EBPbeta (Ser105) Antibody (Rat Specific) #3081

C/EBP   C/EBP-β   C/EBPβ   CCAAT/enhancer binding protein   CEBP   CEBP-β   CHOP   LAP   NF-IL6  

No. Size Price
3081S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3081 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Rat, Endogenous 41 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-C/EBPbeta (Ser105) Antibody (Rat Specific) detects endogenous levels of rat C/EBPbeta only when phosphorylated at serine 105. It does not cross-react with phosphorylated rat C/EBP family members alpha, delta, gamma, epsilon or zeta. It also does not recognize the p20 LIP rat C/EBPbeta isoform.

Phospho-C/EBPβ (Ser105)抗体(兔特异性)识别内源性的Ser105磷酸化的C/EBPβ。此抗体与其它磷酸化的C/EBP亚型α, δ, γ,ε或ζ没有交叉反应。它也不识别p20 LIP大鼠C/EBPβ亚型。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser105 of rat C/EBPbeta. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体用与大鼠C/EBPβ中Ser105位点附近的氨基酸序列对应的人工合成磷酸化的肽段免疫动物制成。该抗体使用蛋白A和肽亲和层析纯化而得。

Western Blotting

Western Blotting

Western blot analysis of extracts from untreated or serum stimulated PC12 cells, using Phospho-C/EBPbeta (Ser105) Antibody (Rat Specific) (A, B) or C/EBPbeta Antibody #3082 (C, D). Nitrocellulose membranes B and D were treated with alkaline phosphatase (CIP) to show the phospho-specificity of Phospho-C/EBPbeta (Ser105) Antibody.

对PC12细胞抽提液(未处理或血清刺激),使用Phospho-C/EBPbeta (Ser105) Antibody (兔特异性) (A, B)或C/EBPbeta Antibody #3082 (C, D)进行Western blot分析。硝酸纤维素膜B和D经过碱性磷酸酶(CIP)处理以显示Phospho-C/EBPbeta (Ser105) Antibody的特异性。

Background

CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors critical for cellular differentiation, terminal functions and inflammatory response (1). Six members of the family have been characterized (C/EBPα, -β, -γ, -δ, -ε and -ζ) and are distributed in a variety of tissues (1). There are two forms of C/EBPβ, the 38 kDa liver activating protein (LAP) and the 20 kDa liver inhibitory protein (LIP) which may be products of alternative translation. The 38 kDa LAP protein is a transcriptional activator while LIP may act as an inhibitor of C/EBPβ transcriptional activity (2). Phosphorylation of C/EBPβ at distinct sites stimulates its transcriptional activity (3-5). Phosphorylation at serine 105 of rat C/EBPβ, a unique site only present in the rat sequence, seems essential for rat C/EBPβ activation (6).

CCAAT/增强子结合蛋白(C/EBP)是一个转录因子家族,对细胞分化、终端功能和炎症反应十分关键(1)。目前已确定该家族的6个成员(C/EBPα, β, δ, γ, ε, ζ),它们分布在不同组织(1)。C/EBPβ有两种形式,38kDa的肝激活蛋白(LAP)和20kDa的肝抑制蛋白(LIP),它们可能是可变翻译的产物。38kDa LAP蛋白是转录激活因子,LIP是C/EBPβ的转录活性的抑制剂(2)。C/EBPβ不同位点的磷酸化刺激其转录活性(3-5)。大鼠C/EBPβ Ser105磷酸化是仅在大鼠序列中出现的一个独特位点,似乎对大鼠C/EBPβ的激活至关重要(6)。

  1. Lekstrom-Himes, J. and Xanthopoulos, K.G. (1998) J. Biol. Chem. 273, 28545-28548.
  2. Calkhoven, C.F. et al. (2000) Genes Dev. 14, 1920-1932.
  3. Wegner, M. et al. (1992) Science 256, 370-373.
  4. Trautwein, C. et al. (1993) Nature 364, 544-547.
  5. Nakajima, T. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 2207-2211.
  6. Buck, M. et al. (1999) Mol. Cell 4, 1087-1092.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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