Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb #3079

No. Size Price
3079S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3079T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
3079 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 48 Rabbit IgG
IF-IC 1:1600

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb detects endogenous levels of Aurora A only when phosphorylated at Thr288. Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb能够检测内源性的苏氨酸(288位点)磷酸化的极光激酶A。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Thr288 of human Aurora A. 该单克隆抗体是有合成的人源的针对极光激酶A苏氨酸(288位)磷酸化肽段免疫动物而生产的。

IF-IC

IF-IC

Confocal immunofluorescent analysis of mitotic HeLa cells during metaphase (left) and anaphase (right) using Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb (red) and Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 激光共聚焦荧光法检测有丝分裂期的HeLa细胞。左图为有丝分裂中期,右图为有丝分裂后期,使用的抗体为 Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb (红色) and Phospho-Histone H3 (Ser10) (6G3) Mouse mAb #9706 (绿色)。蓝色伪彩为DNA荧光染料(产品信息为 DRAQ5®#4084 )

Western Blotting

Western Blotting

Western blot analysis of extracts from hydroxyurea or nocodazole treated HeLa and HT29 cells using Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb. Western blot方法检测羟基脲或者诺考达唑处理的HeLa 和 HT29 细胞提取物,使用的抗体为Phospho-Aurora A (Thr288) (C39D8) Rabbit mAb。

Background

Aurora A (AIK) is a cell cycle-regulated serine/threonine protein kinase that is overexpressed in many tumor cell lines (1-3). Phosphorylation of Aurora A at Thr288 within the kinase activation loop results in a significant increase in its activity and may target the protein for proteasomal degradation during mitosis (4). The closely-related kinase Aurora B (AIM1) has been implicated in multiple mitotic events (5) and siRNA silencing of Aurora B expression results in reduced histone H3 phosphorylation, aberrant chromosome alignment/segregation and altered survivin localization (6). 极光激酶A(AIK)是一种调节细胞周期的丝氨酸/苏氨酸蛋白激酶,在多种肿瘤细胞系中过表达(1-3)。在蛋白激酶激活回路中,极光激酶A苏氨酸(288位)的磷酸化可显著提高其活性并靶向有丝分裂过程中蛋白质的蛋白酶体降解(4)。与极光激酶A密切相关的极光激酶B(AIM1)在多重有丝分裂事件中发挥作用,siRNA沉默其表达之后可以导致组蛋白H3磷酸化减少、异常的染色体排列/分离以及生存蛋白的定位改变(6)。

  1. Bischoff, J.R. et al. (1998) EMBO J 17, 3052-65.
  2. Zhou, H. et al. (1998) Nat Genet 20, 189-93.
  3. Sen, S. et al. (1997) Oncogene 14, 2195-200.
  4. Walter, A.O. et al. (2000) Oncogene 19, 4906-16.
  5. Kallio, M.J. et al. (2002) Curr Biol 12, 900-5.
  6. Hauf, S. et al. (2003) J Cell Biol 161, 281-94.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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