Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-c-Kit (Tyr703) (D12E12) Rabbit mAb #3073

No. Size Price
3073S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3073T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
3073 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 145 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-c-Kit (Tyr703) (D12E12) Rabbit mAb detects endogenous levels of c-Kit only when phosphorylated at Tyr703. This antibody may cross-reacts with other tyrosine-phosphorylated PTKs. 抗磷酸化的-c-Kit (Tyr703))的兔源单克隆抗体(D12E12)仅在Tyr703被磷酸化后才能检测到c-Kit的存在。本抗体也能与其它酪氨酸磷酸化的PTKs发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr703 of human c-Kit. 单克隆抗体通过免疫动物得到,该合成磷酸化多肽是根据人的c-Kit蛋白Tyr703附近的氨基酸序列合成的。

Western Blotting

Western Blotting

Western blot analysis of extracts of H526 cells, untreated or treated with SCF, using Phospho-c-Kit (Tyr703) (D12E12) Rabbit mAb (upper) or c-Kit Antibody #3392 (lower). 用Phospho-c-Kit (Tyr703) (D12E12) Rabbit mAb(上)和c-Kit Antibody #3392(下)对经过和未经SCF刺激的H526细胞的提取物进行免疫印迹检测。


c-Kit is a member of the subfamily of receptor tyrosine kinases that includes PDGF, CSF-1, and FLT3/flk-2 receptors (1,2). It plays a critical role in activation and growth in a number of cell types including hematopoietic stem cells, mast cells, melanocytes, and germ cells (3). Upon binding with its stem cell factor (SCF) ligand, c-Kit undergoes dimerization/oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCγ, the p85 subunit of PI3 kinase, SHP2, and CrkL (4). Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders (5), and mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors (6). Tyr719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo (7).

c-Kit是受体酪氨酸激酶亚族的成员之一,这个家族包扩PDGF、 CSF-1和 FLT3/flk-2受体 (1,2)。它在造血干细胞、肥大细胞、黑色素细胞和生殖细胞等多种细胞的活化和生长过程中起重要作用(3)。通过和配体干细胞因子 (SCF)相结合,c-Kit发生二聚化/寡聚化并自磷酸化。激活的c-Kit能够招募下游含SH2信号传到成分,包括PLCγ、PI3K激酶的p85亚基、 SHP2和 CrkL,并使其酪氨酸磷酸化 (4)。导致c-Kit激酶功能减弱的分子损伤往往和各种发育异常相关(5),而促使c-Kit组成型活化的突变则能导致肥大细胞增多症和胃肠道间质肿瘤(6)。Tyr719位于激酶催化插入区域。Tyr719位点磷酸化的c-Kit 的能够在体内或体外与PI3K激酶的p85亚基结合(7)。

Tyr703 is also located in kinase insert of c-kit. Its phosphorylation provides a docking site for Grb2 binding (8). It was demonstrated that Tyr703 was constitutively phosphorylated in GIST tumor cells (9).

Tyr703定位在c-kit的激酶插入区,它的磷酸化能够为Grb2的结合提供停靠位点(8)。据证明,GIST 肿瘤细胞中Tyr703发生组成型的磷酸化(9)。

  1. Martin, F.H. et al. (1990) Cell 63, 203-11.
  2. Yarden, Y. et al. (1987) EMBO J 6, 3341-51.
  3. Gommerman, J.L. et al. (1997) J Biol Chem 272, 30519-25.
  4. Sattler, M. et al. (1997) J Biol Chem 272, 10248-53.
  5. Nocka, K. et al. (1990) EMBO J 9, 1805-13.
  6. Hirota, S. et al. (1998) Science 279, 577-80.
  7. Blume-Jensen, P. et al. (2000) Nat Genet 24, 157-62.
  8. Thömmes, K. et al. (1999) Biochem J 341 ( Pt 1), 211-6.
  9. Duensing, A. et al. (2004) Oncogene 23, 3999-4006.

Application References

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