Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-PDK1 (Ser241) Antibody #3061

PDK   PKB kinase  

No. Size Price
3061L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
3061S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3061 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 58 to 68 Rabbit
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-PDK1 (Ser241) Antibody detects PDK1 only when phosphorylated at Ser241.

Phospho-PDK1 (Ser241) Antibody能够检测Ser241位点磷酸化的PDK1蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser241 of human PDK1. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是由合成的人源PDK1蛋白Ser241位点周围序列的磷酸化肽段免疫动物而生产的。抗体由protein A和肽亲和层析技术纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells (starved for 16 hours) treated with PDGF (50 ng/ml), using Phospho-PDK1 (Ser241) Antibody (upper) or control PDK1 antibody (lower). The phospho-specificity of the antibody was confirmed by treating the membrane with calf intestinal alkaline phosphatase (CIP) after Western transfer. Western blot 检测分析用 PDGF (50 ng/ml)处理的饥饿16小时的NIH/3T3细胞提取物,使用的抗体是 Phospho-PDK1 (Ser241) Antibody(上图)和control PDK1 antibody(下图)。Western转膜后,用小牛肠碱性磷酸酶 (CIP) 处理膜来确认抗体识别磷酸化蛋白的特异性。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells transiently transfected with DNA constructs expressing Wild-type PDK1 or S241A mutant PDK1, treated with IGF-1, using Phospho-PDK1 (Ser241) Antibody (upper) or control PDK1 Antibody #3062 (lower). (Cell lysates provided by Dr. M. Scheid, Ontario Cancer Institute, University Health Network, Toronto.) Western blot 检测分析293细胞提取物,用表达野生型PDK1和S241A突变的PDK1的DNA载体瞬转后,用IGF-1处理;使用的抗体是 Phospho-PDK1 (Ser241) Antibody (上图)和control PDK1 Antibody #3062(下图)。细胞裂解物由Dr. M. Scheid, Ontario Cancer Institute, University Health Network, Toronto友情赠送。


Phosphoinositide-dependent protein kinase 1 (PDK1) plays a central role in many signal transduction pathways (1,2) including the activation of Akt and the PKC isoenzymes p70 S6 kinase and RSK (3). Through its effects on these kinases, PDK1 is involved in the regulation of a wide variety of processes, including cell proliferation, differentiation and apoptosis.Several serine sites (Ser25, Ser241, Ser393/396 and Ser410) are phosphorylated on PDK1 in unstimulated human embryo kidney 293 cells, as well as IGF-1 stimulated cells (4). Phosphorylation on the activation loop Ser241 by autophosphorylation is necessary for PDK1 activity (4).

磷酸肌醇依赖蛋白激酶1(PDK1)在多种信号传导通路中发挥核心作用(1,2),包括Akt和PKC同工酶p70 S6激酶和RSK的活化(3)。通过这些激酶,PDK1参与调控了广泛的生物学过程,包括细胞增殖,分化和凋亡。在未刺激的人类胚肾293细胞和IGF-1刺激的细胞中,PDK1的几个丝氨酸位点(Ser25, Ser241, Ser393/396 and Ser410)发生磷酸化(4)。在活化环Ser241位点的自磷酸化对于PDK1的活性是必须的(4)。

  1. Belham, C. et al. (1999) Curr. Biol. 9, R93-R96.
  2. Toker, A. and Newton, A.C. (2000) Cell 103, 185-188.
  3. Williams, M.R. et al. (2000) Curr. Biol. 10, 439-448.
  4. Casamayor, A. et al. (1999) Biochem J 342 ( Pt 2), 287-92.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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