Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-Na,K-ATPase α1 (Tyr10) Antibody #3060


No. Size Price
3060S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
3060 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 100 Rabbit
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Rat, Monkey, Bovine, Pig,

Specificity / Sensitivity

Phospho-Na,K-ATPase α1 (Tyr10) Antibody recognizes endogenous levels of Na,K-ATPase α1 only when phosphorylated at Tyr10. The antibody cross-reacts with an induced 75-80 kDa doublet of unknown origin. Phospho-Na,K-ATPase α1 (Tyr10) Antibody检测仅在Tyr10位点磷酸化的内源性Na,K-ATPase α1总蛋白。该抗体与一个诱导的75-80 kDa大小不知来源的成对发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr10 of rat Na,K-ATPase α1. Antibodies are purified using protein A and peptide affinity chromatography. 通过人工合成大鼠Na,K-ATPase α1蛋白Tyr10位点相应的磷酸化片段去免疫动物从而制备多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or vanadate-treated, and A431 cells, untreated or EGF-treated, using Phospho-Na,K-ATPase α1 (Tyr10) Antibody (upper) or total Na,K-ATPase α1 Antibody #3010 (lower). 使用Phospho-Na,K-ATPase α1 (Tyr10) Antibody (上图)和total Na,K-ATPase α1 Antibody #3010 (下图),免疫印迹(Western Blot)分析untreated或vanadate-treated的HeLa细胞和untreated或EGF-treated的A431细胞。


The Na,K-ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. This ion channel uses the energy derived from ATP hydrolysis to maintain membrane potential by driving sodium export and potassium import across the plasma membrane against their electrochemical gradients. It is composed of a catalytic α subunit and a β subunit (reviewed in 1). Several phosphorylation sites have been identified for the α1 subunit. Tyr10 is phosphorylated by an as yet undetermined kinase (2), Ser16 and Ser23 are phosphorylated by PKC, and Ser943 is phosphorylated by PKA (3-5). All of these sites have been implicated in the regulation of enzyme activity in response to hormones and neurotransmitters, altering trafficking and kinetic properties of Na,K-ATPase. Altered phosphorylation in response to angiotensin II stimulates activity in the rat proximal tubule (6). Na,K-ATPase is also involved in other signal transduction pathways. Insulin regulates its localization in differentiated primary human skeletal muscle cells, and this regulation is dependent on ERK1/2 phosphorylation of the α subunit (7). Na,K-ATPase and Src form a signaling receptor complex that affects regulation of Src kinase activity and, subsequently, its downstream effectors (8,9). Na,K-ATPase是一个跨膜异二聚体,其属于P型ATP酶家族。该离子通道使用来自水解ATP释放的能量,通过逆电化学梯度输送Na+到胞外同时摄取K+入胞去维持膜电位。该酶是由一个催化性α亚基和一个β亚基构成(见综述1)。该α1 亚基的数个磷酸化位点已被鉴定。Tyr10位点通过一个尚未确定激酶磷酸化(2),Ser16和Ser23位点通过PKC激酶磷酸化,以及Ser943位点通过PKA位点磷酸化(3-5)。在激素和神经递质刺激下,所有这些位点都涉及调节该酶活性,从而改变Na,K-ATPase的交换和动力性性质。血管紧张素II 改变的磷酸化刺激在大鼠近端小管的活性(6)。Na,K-ATPase也涉及其他信号转导通路。胰岛素调节在分化的主要人源骨骼肌细胞的定位,并且该调节依赖于α 亚基的ERK1/2磷酸化(7)。 Na,K-ATPase和Src形成一个信号受体复合物,该复合物影响Src激酶活性的调节,以及随后它的下游效应分子(8,9)。

  1. Therien, A.G. and Blostein, R. (2000) Am J Physiol Cell Physiol 279, C541-66.
  2. Féraille, E. et al. (1999) Mol Biol Cell 10, 2847-59.
  3. Fisone, G. et al. (1994) J Biol Chem 269, 9368-73.
  4. Feschenko, M.S. and Sweadner, K.J. (1995) J Biol Chem 270, 14072-7.
  5. Beguin, P. et al. (1994) J Biol Chem 269, 24437-45.
  6. Yingst, D.R. et al. (2004) Am J Physiol Renal Physiol 287, F713-21.
  7. Al-Khalili, L. et al. (2004) J Biol Chem 279, 25211-8.
  8. Tian, J. et al. (2006) Mol Biol Cell 17, 317-26.
  9. Liang, M. et al. (2006) J Biol Chem 281, 19709-19.

Application References

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