Cell Signaling Technology

Product Pathways - NF-kB Signaling

Acetyl-NF-κB p65 (Lys310) Antibody #3045

nfκb p65  

No. Size Price
3045S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3045 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Transfected Only 65 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Rat, Monkey, Bovine, Dog,

Specificity / Sensitivity

Acetyl-NF-κB p65 (Lys310) Antibody detects transfected levels of NF-κB only when acetylated at Lys310.

Acetyl-NF-κB p65 (Lys310)抗体只能检测转染水平的在Lys310位点乙酰基化的NF-κB蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic acetylated peptide corresponding to residues surrounding Lys310 of NF-κB. Antibodies were purified by protein A and peptide affinity chromatography.

此多克隆抗体是通过合成对应的NF-κB Lys310位点周围乙酰基化得肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, mock transfected or transfected with NF-κB or NF-κB plus CBP, using Acetyl-NF-κB (Lys310) Antibody. Western blots were competed with acetylated peptide (middle) or non-acetylated peptide (right).Western免疫印迹。用 Acetyl-NF-κB (Lys310) Antibody研究对照转染和转染了NF-κB 或 NF-κB 加上CBP的 HeLa 细胞的细胞提取液。Western免疫印迹通过与乙酰基化得多肽(中间图) 竞争或未乙酰基化的多肽 (右图)竞争。


Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11).

核因子κ B(NF-κB)/Rel 家族的转录调控因子在炎症反应和免疫反应中发挥了至关重要的作用(1,2)。在哺乳动物中一共有5个家族成员: RelA, c-Rel, RelB, NF-κB1 (p105/p50) 和 NF-κB2 (p100/p52)。 p105 和 p100 在蛋白水解酶的作用下分别形成p50 和 p52。Rel与p50和p52形成二聚体,此复合体能够结合到DNA上调控转录。在未刺激的状态下, NF-κB 在IκB抑制剂作用下在细胞质中处于非活性状态(3-5)。 NF-κB激活因子能够诱导 IκB 蛋白的磷酸化, 这就使IκB能够快速的经过泛素化-蛋白酶通路降解从而释放 NF-κB,激活的NF-κB入核调控基因的表达(6-8)。 NIK 和 IKKα (IKK1) 调节磷酸化并促使NF-κB2 (p100) 生成p52, p52之后会入核发挥功能(9-11)。

NF-κB assembly with IκB, as well as its DNA binding and transcriptional activity, are regulated by p300/CBP acetytransferases that principally target Lys218, Lys221 and Lys310 (12-14). This process is reciprocally regulated by histone deacetylases (HDACs); several HDAC inhibitors have been shown to activate NF-κB (12-14).

NF-κB 与IκB组装,同时还有 DNA 结合和转录活性, 通过p300/CBP乙酰基转移酶调控,其目标是 Lys218, Lys221 和 Lys310 (12-14)。此过程受到组蛋白乙酰基酶(HDACs)相反的调控作用;几个HDAC抑制因子被发现能够激活NF-κB (12-14)。

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  2. Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20.
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  4. Thompson, J.E. et al. (1995) Cell 80, 573-82.
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  7. Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63.
  8. Chen, Z.J. et al. (1996) Cell 84, 853-62.
  9. Senftleben, U. et al. (2001) Science 293, 1495-9.
  10. Coope, H.J. et al. (2002) EMBO J 21, 5375-85.
  11. Xiao, G. et al. (2001) Mol Cell 7, 401-9.
  12. Ashburner, B.P. et al. (2001) Mol. Cell. Biol. 21, 7065-7077.
  13. Mayo, M.W. et al. (2003) J. Biol. Chem. 278, 18980-1899.
  14. Chen, L.F. et al. (2002) EMBO J. 21, 6539-6548.

Application References

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