Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-MAPKAPK-2 (Thr334) Antibody #3041

map   mapk   mapkap   mapkap 2   mapkap-2   mapkap2   mapkapk 2   mapkapk-2   mapkapk2   mk 2   mk-2   mk2  

No. Size Price
3041S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
3041 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 49 Rabbit
IHC-P 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

Phospho-MAPKAPK-2 (Thr334) Antibody detects endogenous levels of MAPKAPK-2 protein phosphorylated at threonine 334. It may also cross-react with MAPKAPK-3 phosphorylated at threonine 313.

Phospho-MAPKAPK-2 (Thr334) Antibody兔多抗能检测内源性Thr334位点磷酸化的MAPKAPK-2蛋白水平。该抗体可能会与Thr313位点磷酸化的MAPKAPK-3蛋白发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr334 of human MAPKAPK-2. Antibodies are purified by protein A and peptide affinity chromatography.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma showing, nuclear localization, using Phospho-MAPKAPK-2 (Thr334) Antibody.免疫组织化学方法检测石蜡包埋的人乳腺癌组织,显示细胞核位置。使用的抗体是Phospho-MAPKAPK-2 (Thr334) Antibody。

Western Blotting

Western Blotting

Western blot analysis of extracts from untreated or UV treated HeLa and Cos cells, using Phospho-MAPKAPK-2 (Thr334) Antibody (upper) or MAPKAPK-2 Antibody #3042 (lower).Western blot方法检测细胞提取物:未经处理的和紫外照射的HeLa、COS细胞,使用的抗体是Phospho-MAPKAPK-2 (Thr334) Antibody (上图)和 MAPKAPK-2 Antibody #3042 (下图)。


In response to cytokines, stress and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK (1). Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272 and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay (2). Phosphorylation at Thr222, Ser272 and Thr334 appears to be essential for the activity of MAPKAPK-2 (2). Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2 (2).

在细胞因子、压力和化学因子的刺激下,丝裂原活化蛋白激酶激活的蛋白激酶2(MAPKAPK-2)能够迅速被磷酸化并被激活。研究显示MAPKAPK-2是p38 MAPK的直接靶点(1)。在体内,在压力应激下MAPKAPK-2的多个氨基酸都能被磷酸化。但是,在体外激酶实验中,仅有4个位点(Thr25, Thr222, Ser272, 和 Thr334)能够被p38 MAPK磷酸化(2)。Thr222, Ser272和Thr334的磷酸化对于MAPKAPK-2的激酶活性是必须的(2)。Thr25可以在体外被p42 MAPK磷酸化,但是该磷酸化对于MAPKAPK-2的激活不是必须的(2)。

  1. Rouse, J. et al. (1994) Cell 78, 1027-1037.
  2. Ben-Levy, R. et al. (1995) EMBO J. 14, 5920-5930.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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