Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-MYPT1 (Ser507) Antibody #3040


No. Size Price
3040S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3040T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
3040 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 140 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Monkey,

Specificity / Sensitivity

MYPT1 (Ser507) Antibody detects endogenous levels of MYPT1 only when phosphorylated at Ser507.

MYPT1 (Ser507) Antibody仅检测Ser507位点磷酸化的内源性MYPT1蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser507 of human MYPT1. Antibodies are purified using protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and Jurkat cells, untreated or treated with calyculin A #9902, using Phospho-MYPT1 (Ser507) Antibody (upper) or MYPT1 Antibody #2634 (lower).

使用Phospho-MYPT1 (Ser507) Antibody (上图)和MYPT1 Antibody #2634 (下图),免疫印迹(Western Blot)分析HeLa和Jurkat细胞中Phospho-MYPT1 (Ser507)和MYPT1蛋白水平。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or treated with Calyculin A #9902, using Phospho-MYPT1 (Ser507) Antibody. The phospho-specificity of the antibody was verified by peptide blocking using no peptide (left), phospho-Ser507 peptide (middle) or phospho-Ser668 peptide (right).

使用Phospho-MYPT1 (Ser507) Antibody,免疫印迹(Western Blot)分析HeLa细胞中Phospho-MYPT1 (Ser507)蛋白水平,细胞分为未处理组或Calyculin A #9902处理组。使用no peptide (左图)、phospho-Ser507 peptide (中图)或phospho-Ser668 peptide (右图)通过多肽封闭证明该抗体的磷酸化特异性。


Protein phosphatase 1 (PP1) is a ubiquitous eukaryotic protein serine/threonine phosphatase involved in the regulation of various cell functions. Substrate specificity is determined by the binding of a regulatory subunit to the PP1 catalytic subunit (PP1c). It is estimated that over fifty different regulatory subunits exist (1). 
 The myosin phosphatase holoenzyme is composed of three subunits: the PP1 catalytic subunit (PP1c), a targeting/regulatory subunit (MYPT/myosin-binding subunit of myosin phosphatase) and a 20 kDa subunit of unknown function (M20). MYPT binding to PP1cδ alters the conformation of the catalytic cleft and increases enzyme activity and specificity (2). Two MYPT isoforms that are 61% identical have been described. MYPT1 is widely expressed while MYPT2 expression appears to be exclusive to heart and brain (3). Related family members also include MBS85, MYPT3 and TIMAP (4). 
 Myosin phosphatase regulates the interaction of actin and myosin in response to signaling through the small GTPase Rho. Rho activity inhibits myosin phosphatase via Rho-associated kinase (ROCK). Phosphorylation of MYPT1 at Thr696 and Thr853 results in phosphatase inhibition and cytoskeletal reorganization (5,6).

Protein phosphatase 1 (PP1)是一个普遍存在的真核蛋白质丝氨酸/苏氨酸磷酸酶,其涉及不同细胞功能的调节。底物的特异性通过一个调节亚单位与PP1 catalytic subunit (PP1c)的结合被确定。据估计大约有五十个不同的调节亚单位存在(1)。myosin 磷酸酶全酶有三个亚单位组成:PP1催化亚单位(PP1c) 、一个靶向/调节亚单位(MYPT/myosin-binding subunit of myosin phosphatase)和一个未知功能的20 kDa大小的亚单位 (M20)。MYPT蛋白结合到PP1cδ蛋白可改变催化部位的构象以及子女国家酶活性和特异性 (2)。61%序列相同的两个MYPT亚单位已经被描述。MYPT1蛋白广泛表达,而MYPT2 蛋白的表达只表达在心脏和大脑组织(3)。相关的家族成员也诱导MBS85、MYPT3和TIMAP (4)。在小GTPase Rho的信号下,Myosin磷酸酶调节actin和myosin相互作用。通过Rho-associated kinase (ROCK)作用,Rho活性抑制myosin磷酸酶。MYPT1蛋白在Thr696和Thr853位点的磷酸化导致磷酸酶抑制和细胞骨架重组(5,6)。

Phospho-MYPT1 (Ser507) Antibody is directed at a site that was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for modification site discovery. Phosphorylation at Ser507 was discovered using an Akt substrate antibody. Please visit PhosphoSitePlus®, CST's modification site knowledgebase, at www.phosphosite.org for more information.

Phospho-MYPT1 (Ser507) Antibody直接作用一个位点,该位点磷酸化被鉴定 ,通过Cell Signaling Technology (CST)公司使用PhosphoScan®, a CST™ LC-MS/MS平台发现修饰的位点(5)。Ser507位点的磷酸化使用一个Akt底物抗体被发现。请访问PhosphoSitePlus®, CST's修饰位点知识库,更多的信息查询网站:www.phosphosite.org。

  1. Cohen, P.T. (2002) J Cell Sci 115, 241-56.
  2. Terrak, M. et al. (2004) Nature 429, 780-4.
  3. Fujioka, M. et al. (1998) Genomics 49, 59-68.
  4. Ito, M. et al. (2004) Mol Cell Biochem 259, 197-209.
  5. Birukova, A.A. et al. (2004) Microvasc Res 67, 64-77.
  6. Birukova, A.A. et al. (2004) J Cell Physiol 201, 55-70.

Application References

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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