Cell Signaling Technology

Product Pathways - Metabolism

Phospho-Insulin Receptor β (Tyr1345) (14A4) Rabbit mAb #3026

I-R   IR   IR-beta  

No. Size Price
3026S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3026 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Transfected Only 95 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-Insulin Receptor (Tyr1345) (14A4) Rabbit mAb detects transfected levels of insulin receptor β only when phosphorylated at Tyr1345. The antibody slightly cross-reacts with activated IGF-I receptors and some other activated tyrosine kinases.

Phospho-Insulin Receptor (Tyr1345) (14A4)兔单克隆抗体可识别转染的Tyr1345磷酸化的胰岛素受体β。它与激活的IGF-I受体和其它激活的酪氨酸激酶有轻度交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1345 of human insulin receptor β.

该单克隆抗体用与人类胰岛素受体β蛋白中Tyr1345位点附近的氨基酸序列对应的人工合成磷酸化肽段免疫动物制成。

Western Blotting

Western Blotting

Western blot analysis of CHO IR/IRS-1 cells overexpressing human insulin receptor, untreated or treated with insulin (100 nM for 5 min), using Phospho-Insulin Receptor β (Tyr1345) (14A4) Rabbit mAb (upper) or Insulin Receptor β (4B8) Rabbit mAb #3025 (lower).

对过表达人胰岛素受体的IR/IRS-1 细胞(未处理,或100nM胰岛素处理5分钟),使用Phospho-Insulin Receptor β (Tyr1345) (14A4) Rabbit mAb兔单抗(上图)或Insulin Receptor β (4B8) Rabbit mAb兔单抗 #3025(下图)进行Western blot分析。

Background

Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8).I型胰岛素样生长因子受体(IGF-I)是一种跨膜受体酪氨酸激酶,广泛表达于许多细胞系,并在胎儿和产后组织细胞中表达(1-3)。受体与IGF-I和IGF-Ⅱ配体结合后发生自身磷酸化。 激酶结构域的三个酪氨酸残基(Tyr1131,Tyr1135,Tyr1136)是最早确定的三个主要磷酸化位点(4)。三个酪氨酸残基的磷酸化对激酶的活化是必要的(5,6)。胰岛素受体(IR)与IGF-I受体有显著的结构和功能的相似性,都有激酶结构域激活环中对应的酪氨酸残基(Tyr1146/1150/1151)的存在。IR的酪氨酸磷酸化是胰岛素刺激最早的细胞反应之一(7)。自身磷酸化从Tyr1146和Tyr1150或Tyr1151的磷酸化开始,但完全的激酶活性需要三个酪氨酸残基的磷酸化(8)。

  1. Adams, T.E. et al. (2000) Cell Mol Life Sci 57, 1050-93.
  2. Baserga, R. (2000) Oncogene 19, 5574-81.
  3. Scheidegger, K.J. et al. (2000) J Biol Chem 275, 38921-8.
  4. Hernández-Sánchez, C. et al. (1995) J Biol Chem 270, 29176-81.
  5. Lopaczynski, W. et al. (2000) Biochem Biophys Res Commun 279, 955-60.
  6. Baserga, R. (1999) Exp Cell Res 253, 1-6.
  7. White, M.F. et al. (1985) J Biol Chem 260, 9470-8.
  8. White, M.F. et al. (1988) J Biol Chem 263, 2969-80.

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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