Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb #3007

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No. Size Price
3007L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
3007S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3007T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
3007 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 49 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb detects endogenous levels of MAPKAPK-2 protein only when phosphorylated at threonine 334.

Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb兔单抗能检测内源性Thr334位点磷酸化的MAPKAPK-2蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr334 of human MAPKAPK-2.

该单克隆抗体是采用合成的与人源MAPKAPK-2蛋白Thr334位点周围残基相一致的磷酸化肽段免疫动物后获得的。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing nuclear localization, using Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb.免疫组织化学方法检测石蜡包埋的人结肠癌组织,显示细胞核的位置,使用的抗体是Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, untreated (left) or λ-phosphatase-treated (right), using Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb.免疫组织化学方法检测石蜡包埋的未处理(左侧)和λ-磷酸酶处理(右侧)的人肺癌组织。使用的抗体是Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb in the presence of control peptide (left) or Phospho-MAPKAPK-2 (Thr334) Blocking Peptide #1041 (right).免疫组织化学方法检测石蜡包埋的人结肠癌组织。使用的抗体是Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb。左侧是对照组肽段,右侧是Phospho-MAPKAPK-2 (Thr334) Blocking Peptide #1041。

Western Blotting

Western Blotting

Western blot analysis of extracts from untreated or UV+TPA-treated HeLa and COS cells, using Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb (upper), or MAPKAPK-2 Antibody #3042 (lower).Western blot方法检测细胞提取物:未经处理的和UV+TPA处理的HeLa、COS细胞,使用的抗体是Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb (上图)和MAPKAPK-2 Antibody #3042 (下图)。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells, untreated (blue) or UV-treated (green), using Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb.Flow cytometric方法检测细胞提取物:未处理(蓝色)和紫外照射(绿色)的HeLa细胞,使用的抗体是Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, untreated (left), UV-treated (center) or UV and lambda phosphatase-treated (right), using Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).激光共聚焦荧光法检测:未经处理的(左侧)、紫外照射的(中间)和UV+lambda 磷酸酶处理的(右侧)HeLa 细胞。使用的抗体是Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb (绿色),肌动蛋白纤维素已被DY-554 phalloidin (红色)标记。

Background

In response to cytokines, stress and chemotactic factors, MAP kinase-activated protein kinase 2 (MAPKAPK-2) is rapidly phosphorylated and activated. It has been shown that MAPKAPK-2 is a direct target of p38 MAPK (1). Multiple residues of MAPKAPK-2 are phosphorylated in vivo in response to stress. However, only four residues (Thr25, Thr222, Ser272 and Thr334) are phosphorylated by p38 MAPK in an in vitro kinase assay (2). Phosphorylation at Thr222, Ser272 and Thr334 appears to be essential for the activity of MAPKAPK-2 (2). Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2 (2).Thr25 is phosphorylated by p42 MAPK in vitro, but is not required for the activation of MAPKAPK-2 (2).

在细胞因子、压力和化学因子的刺激下,丝裂原活化蛋白激酶激活的蛋白激酶2(MAPKAPK-2)能够迅速被磷酸化并被激活。研究显示MAPKAPK-2是p38 MAPK的直接靶点(1)。在体内,在压力应激下MAPKAPK-2的多个氨基酸都能被磷酸化。但是,在体外激酶实验中,仅有4个位点(Thr25, Thr222, Ser272, 和 Thr334)能够被p38 MAPK磷酸化(2)。Thr222, Ser272和Thr334的磷酸化对于MAPKAPK-2的激酶活性是必须的(2)。Thr25可以在体外被p42 MAPK磷酸化,但是该磷酸化对于MAPKAPK-2的激活不是必须的(2)。

  1. Rouse, J. et al. (1994) Cell 78, 1027-1037.
  2. Ben-Levy, R. et al. (1995) EMBO J. 14, 5920-5930.

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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