Cell Signaling Technology

Product Pathways - DNA Damage

Phospho-p95/NBS1 (Ser343) Antibody #3001

NBS1   nibrin   p95   RSK1  

No. Size Price
3001S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
3001T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
3001 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Mink, Endogenous 95 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-p95/NBS1 (Ser343) Antibody detects endogenous levels of p95/NBS1 only when phosphorylated at serine 343. Phospho-p95/NBS1 (Ser343) Antibody能够检测内源性丝氨酸(343位)磷酸化的p95/NBS1 蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser343 of human p95/NBS1. Antibodies are purified by protein A and peptide affinity chromatography. 该多克隆抗体是由合成的人源的针对p95/NBS1蛋白丝氨酸(343位)的磷酸化肽段免疫动物,利用蛋白A和多肽亲和层析技术生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from MvILu cells treated with UV or hydroxyurea (HU) for the indicated times, using Phospho-p95/NBS1 (Ser343) Antibody. western blot方法检测不同时间点,由紫外和羟基脲(HU)分别处理的MvILu细胞提取物,使用的抗体为Phospho-p95/NBS1 (Ser343) Antibody。


The Nijmegen breakage syndrome (NBS) is characterized by defects in cell cycle checkpoints, growth retardation, an increased propensity for cancer and sensitivity to ionizing radiation (1). Repair of DNA double-strand breaks by radiation is dependent on a multifunctional complex containing Rad50, Mre11 and the NBS1 gene product p95/NBS1 (also called p95 or nibrin) (2). p95/NBS1 is a protein with a forkhead-associated domain and a carboxy-terminal repeat frequently found in cell cycle regulatory and DNA repair proteins (1,3). The overlap between clinical and cellular phenotypes in ataxia telangiectasia (AT) and NBS suggests that AT-mutated (ATM) and p95/NBS1 function in the same biochemical pathway. ATM interacts with and phosphorylates p95/NBS1 at Ser343 after exposure to ionizing radiation (4-7). Nijmegen 断裂综合征(NBS)的主要特征有细胞周期检验点缺陷,生长迟缓,癌症倾向和离子辐射敏感性增强(1)。辐射修复DNA双链断裂依赖于多功能复合体,包括Rad50, Mre11和NBS1基因产物p95/NBS1 (也称作 p95 或nibrin) (2)。p95/NBS1是一种在细胞周期调控和DNA修复中发现的一种蛋白质,具有叉头相关结构域和重复序列的羧基末端(1,3)。共济失调毛细血管扩张症和NBS临床上以及细胞表型之间的重叠表明ATM和p95/NBS1在同一个生化通路中起作用。ATM和p95/NBS1相互作用并能够在暴露电离辐射之后磷酸化p95/NBS1蛋白丝氨酸343位(4-7)。

  1. Chrzanowska, K.H. et al. (2012) Orphanet J Rare Dis 7, 13.
  2. Lee, J.H. et al. (2013) J Biol Chem 288, 12840-51.
  3. Hari, F.J. et al. (2010) EMBO Rep 11, 387-92.
  4. Wang, H. et al. (2013) PLoS Genet 9, e1003277.
  5. Zhao, S. et al. (2000) Nature 405, 473-7.
  6. Wen, J. et al. (2013) Oncogene 32, 4448-56.

Application References

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