Cell Signaling Technology

Product Pathways - Protein Translation

Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb #2997

40 kDa AKT substrate   40 kDa AKT substrate 1   40 kDa proline-rich AKT substrate 1   AKT1 substrate   AKT1 substrate 1   AKT1S   AKT1S1   AKTS   AKTS1   pras   pras40   Proline-rich AKT1 substrate   Proline-rich AKT1 substrate 1  

No. Size Price
2997S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2997T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
2997 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 40 Rabbit IgG
IP 1:50
IHC-P 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb detects endogenous levels of PRAS40 protein only when phosphorylated at Thr246.

Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb能够检测内源性的Thr246位点磷酸化的PRAS40蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence surrounding Thr246 of human PRAS40.

该单克隆抗体是由合成的人源的针对PRAS40蛋白Thr246位点的磷酸化肽段免疫动物而生产的。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, control (left) or λ phosphatase-treated (right), using Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb.

免疫组织化学方法检测石蜡包埋的人乳腺癌组织,对照(左图)和λ磷酸酶处理(右图);使用的抗体是Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb兔单抗。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb in the presence of control peptide (left) or antigen specific peptide (right).

免疫组织化学方法检测石蜡包埋的人乳腺癌组织,对照肽处理(左图)和抗原特异性肽处理(右图);使用的抗体是Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb兔单抗。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded metastatic SKOV-3 tumor in mouse lung using Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb.

免疫组织化学方法检测石蜡包埋的鼠肺转移的SKOV-3肿瘤组织,使用的抗体是Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb兔单抗。

Western Blotting

Western Blotting

Western blot analysis of extracts from serum starved H3255, Mkn45 and NIH/3T3 cells, untreated or treated with either Iressa (1 μM, 3 hours), Su11274 (1 μM, 3 hours) or insulin (150 nM, 15 minutes), using Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb (upper) or PRAS40 (D23C7) Rabbit mAb #2691 (lower).

Western blot检测分析血清饥饿的H3255, Mkn45 和NIH/3T3 细胞提取物。未处理或者Iressa (1 μM, 3 小时), Su11274 (1 μM, 3 小时) 或 insulin (150 nM, 15 分钟) ;使用抗体是Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb兔单抗 (上图)和 PRAS40 (D23C7) Rabbit mAb兔单抗 #2691(下图)。

Western Blotting

Western Blotting

Western blot analysis of extracts from serum starved HeLa cells, untreated or treated with insulin (100 nM, 5 minutes) or with insulin and λ phosphatase, using Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb (upper) or PRAS40 Antibody #2610 (lower).

Western blot检测分析血清饥饿的Hela细胞提取物。未处理和胰岛素 (100 nM)处理5分钟或胰岛素和 λ磷酸酶处理的;使用抗体是Phospho-PRAS40 (Thr246) (C77D7) Rabbit mAb 兔单抗(上图)和 PRAS40 Antibody #2610 (下图)。

Background

Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt (1,2). One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 protein (2). PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex. Inhibition of mTOR signaling stimulates PRAS40 binding to mTOR, which in turn inhibits mTOR activity (3). PRAS40 interacts with Raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1 (4). mTORC1 in turn phosphorylates PRAS40 at Ser183 (5).

许多生长因子和激素诱导PI3K激酶信号通路,从而激活下游效应蛋白,例如丝氨酸/苏氨酸激酶Akt(1,2)。一个已知的Akt的底物是一个40 kDa的,富含脯氨酸的蛋白(PRAS40),可以结合14-3-3蛋白(2)。PRAS40还能够结合mTOR,传递Akt信号到mTOR复合物。抑制mTOR信号可以刺激PRAS40结合到mTOR上,反过来抑制mTOR的活性(3)。在缺失胰岛素的细胞内PRAS40和mTOR复合物1(mTORC1)中的Raptor 结合,进而抑制细胞周期蛋白Rheb调节的mTORC1信号通路的激活。Akt磷酸化PRAS40的Thr246位点,解除PRAS40对mTORC1的抑制(4)。反过来mTORC1磷酸化PRAS40的Ser183(5)。

  1. Cantley, L.C. (2002) Science 296, 1655-7.
  2. Kovacina, K.S. et al. (2003) J Biol Chem 278, 10189-94.
  3. Vander Haar, E. et al. (2007) Nat Cell Biol 9, 316-23.
  4. Sancak, Y. et al. (2007) Mol Cell 25, 903-15.
  5. Oshiro, N. et al. (2007) J Biol Chem 282, 20329-39.

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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