Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

SET8 (C18B7) Rabbit mAb #2996

No. Size Price
2996S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
2996T 20 µl ( 2 western blots ) ¥1,200.00 现货查询 购买询价 防伪查询
2996 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 43 Rabbit IgG
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),


Species predicted to react based on 100% sequence homology: Bovine, Pig, Horse,

Specificity / Sensitivity

SET8 (C18B7) Rabbit mAb detects endogenous levels of total SET8 protein (both isoforms).

SET8 (C18B7) Rabbit mAb兔单抗能够检测内源性SET8总蛋白水平(两个亚型)。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to human SET8 protein. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from HCT116 and RAW cells using SET8 (C18B7) Rabbit mAb.

使用SET8 (C18B7) Rabbit mAb,免疫印迹(Western blot)分析HCT116和RAW细胞中SET8 (C18B7)的蛋白水平。



Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with 0.5% Triton X-100 for 10 minutes prior to fixation according to Tardat et al. (2007) J. Cell Biol. 179, 1413-26 (right), using SET8 (C18B7) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

使用SET8 (C18B7) Rabbit mAb (绿色),共聚焦免疫荧光分析HeLa细胞,细胞分为未处理 (左图)或在固定之前处理0.5% Triton X-100 for 10 minutes依据Tardat et al. (2007) J. Cell Biol. 179, 1413-26 (右图)。DY-554 phalloidin标记微丝蛋白(红色)。蓝色= DRAQ5™ (DNA荧光染料)。


SET domain-containing lysine methyltransferase 8 (SET8), also known as PR/SET domain-containing protein 7 (PR/SET7), is a member of a family of histone lysine methyltransferases, each of which contains a conserved catalytic SET domain originally identified in Drosophila Su[var]3-9, Enhancer of zeste, and Trithorax proteins (1-3). SET8 is a single-subunit enzyme that mono-methylates histone H4 on Lys20, preferably on nucleosomal substrates (1-3). SET8 protein levels and Histone H4 Lys20 methylation are cell cycle regulated, both increasing in S phase and peaking at G2/M phase (4,5). SET8 interacts with the PCNA protein, associates with sites of active DNA synthesis and is required for DNA replication and genome stability during S phase (5-7). Inhibition of SET8 using shRNA results in arrest of replication forks, induction of double-stranded DNA breaks and a Chk1-mediated cell-cycle arrest in S and G2/M phases of the cell cycle (6,7). Furthermore, SET8 methylates p53 on Lys382, down regulating the pro-apoptotic and checkpoint activation functions of p53 (8). In response to DNA damage, SET8 expression levels decrease, allowing p53 to activate checkpoints and/or apoptosis (8). Both the methylation of histone H4 Lys20 and p53 appear to be important for the functions of SET8 in S phase.

SET domain-containing lysine methyltransferase 8 (SET8)也称为PR/SET domain-containing protein 7 (PR/SET7),它是一个组蛋白赖氨酸甲基转移酶家族成员,它们中每一个都包含一个保守催化的SET结构,该结构起初被鉴定在Drosophila Su[var]3-9、Enhancer of zeste和Trithorax蛋白中(1-3)。SET8是一个单亚单位激酶,它能使histone H4蛋白Lys20位点发生单甲基化,更适应在核小体结构底物上(1-3)。SET8蛋白水平和Histone H4蛋白Lys20位点甲基化是被细胞周期调节的,在S期处于增长期而到G2/M期达到最高峰(4,5)。SET8与PCNA蛋白相互作用,这与活化的DNA合成的位点有关联,并且对于在S期中DNA复制和基因组稳定性是需要的(5-7)。使用shRNA进行SET8的抑制可导致复制叉的阻止、双链DNA诱导的中断和在细胞周期的S和G2/M期中一个Chk1介导的细胞周期停滞 (6,7)。更进一步,SET8可使p53蛋白在Lys382位点甲基化,这可下调p53蛋白促凋亡和监测点激活功能(8)。在DNA损伤反应下,SET8表达水平下降,这允许p53去激活检测点和/或凋亡(8)。histone H4蛋白Lys20位点的甲基化和p53出现对于在S期中SET8的功能是非常重要的。

  1. Fang, J. et al. (2002) Curr Biol 12, 1086-99.
  2. Xiao, B. et al. (2005) Genes Dev 19, 1444-54.
  3. Couture, J.F. et al. (2005) Genes Dev 19, 1455-65.
  4. Rice, J.C. et al. (2002) Genes Dev 16, 2225-30.
  5. Huen, M.S. et al. (2008) J Biol Chem 283, 11073-7.
  6. Tardat, M. et al. (2007) J Cell Biol 179, 1413-26.
  7. Jørgensen, S. et al. (2007) J Cell Biol 179, 1337-45.
  8. Shi, X. et al. (2007) Mol Cell 27, 636-46.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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