Cell Signaling Technology

Product Pathways - Protein Translation

Phospho-mTOR (Ser2448) (49F9) Rabbit mAb (IHC Specific) #2976

FRAP   mammalian target of rapamycin   mTOR   RAFT   Rapamycin   target of rapamycin   TOR  

No. Size Price
2976L 300 µl ( 150 sections ) ¥9,325.00 现货查询 购买询价
2976S 100 µl ( 50 sections ) ¥3,900.00 现货查询 购买询价
2976 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
IHC-P 1:100 Human, Endogenous 289 Rabbit IgG
IHC-F 1:100

Species cross-reactivity is determined by western blot.

Applications Key: IHC-P=Immunohistochemistry (Paraffin), IHC-F=Immunohistochemistry (Frozen),

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-mTOR (Ser2448) (49F9) Rabbit mAb detects endogenous levels of mTOR protein only when phosphorylated at Ser2448.

Phospho-mTOR (Ser2448) (49F9) Rabbit mAb能够检测内源性的Ser2448磷酸化的mTOR蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser2448 of human mTOR.

该单克隆抗体是由合成的人源的针对mTOR蛋白丝氨酸(2448位点)的磷酸化肽段免疫动物而生产的。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-mTOR (Ser2448) (49F9) Rabbit mAb (IHC Specific).

免疫组织化学方法检测石蜡包埋的人乳腺癌组织,使用抗体为 Phospho-mTOR (Ser2448) (49F9) Rabbit mAb兔单抗 (IHC Specific)。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma of the bladder using Phospho-mTOR (Ser2448) (49F9) Rabbit mAb (IHC Specific) in the presence of control peptide (left) or Phospho-mTOR (Ser 2448) Blocking Peptide #1230 (right).

免疫组织化学方法检测石蜡包埋的人膀胱移行上皮癌组织,左图为control peptide处理的,右图为Phospho-mTOR (Ser2448) Blocking Peptide #1230处理的,使用抗体为 Phospho-mTOR (Ser2448) (49F9) Rabbit mAb 兔单抗(IHC Specific) 。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma untreated (left) or lambda phosphatase-treated (right), using Phospho-mTOR (Ser2448) (49F9) Rabbit mAb (IHC Specific).

免疫组织化学方法检测石蜡包埋的人结肠癌组织,左图为未处理的,右图为lambda phosphatase处理的,使用抗体为 Phospho-mTOR (Ser2448) (49F9) Rabbit mAb 兔单抗 (IHC Specific)。

IHC-F (frozen)

IHC-F (frozen)

Immunohistochemical analysis of frozen H1650 xenograft, showing cytoplasmic localization, using Phospho-mTOR (Ser2448) (49F9) Rabbit mAb (IHC specific).

免疫组织化学方法检测冰冻H1650异种移植物组织,展示胞质定位,使用抗体为 Phospho-mTOR (Ser2448) (49F9) Rabbit mAb 兔单抗(IHC Specific) 。

Background

The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9).

哺乳动物雷帕霉素(rapamycin)靶蛋白(mTOR,FRAP,RAFT)是一种丝氨酸/苏氨酸蛋白激酶(1-3),其作为ATP和氨基酸的传感器,可以有效调控营养供应及细胞的生长(4,5)。当营养充分时,mTOR响应磷脂酸介导的信号,进而正向调节p70 S6 激酶,并参与失活eIF4E的抑制剂4E-BP1。这些信号导致特定的mRNA亚群的翻译。mTOR可以被Akt信号通路的PI3K激酶磷酸化修饰Ser2448位点,也能自我磷酸化Ser2481位点(7,8)。mTOR的在细胞生长和动态平衡中起着关键作用,在肿瘤中可能存在调控失常。因此,目前很多研究将mTOR作为一个潜在的抗肿瘤治疗靶点(9)。

  1. Sabers, C.J. et al. (1995) J Biol Chem 270, 815-22.
  2. Brown, E.J. et al. (1994) Nature 369, 756-8.
  3. Sabatini, D.M. et al. (1994) Cell 78, 35-43.
  4. Gingras, A.C. et al. (2001) Genes Dev 15, 807-26.
  5. Dennis, P.B. et al. (2001) Science 294, 1102-5.
  6. Fang, Y. et al. (2001) Science 294, 1942-5.
  7. Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31.
  8. Peterson, R.T. et al. (2000) J Biol Chem 275, 7416-23.
  9. Huang, S. and Houghton, P.J. (2003) Curr Opin Pharmacol 3, 371-7.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications