Cell Signaling Technology

Product Pathways - Protein Translation

Phospho-mTOR (Ser2448) Antibody #2971

FKBP-rapamycin-associated protein   FRAP   mTOR   rapamycin   rapamycin target protein   TOR  

No. Size Price
2971L 300 µl ( 30 western blots ) ¥8,992.00 现货查询 购买询价 防伪查询
2971S 100 µl ( 10 western blots ) ¥3,960.00 现货查询 购买询价 防伪查询
2971 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 289 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Horse,

Specificity / Sensitivity

Phospho-mTOR (Ser2448) Antibody detects endogenous levels of mTOR only when phosphorylated at Ser2448.

Phospho-mTOR (Ser2448) Antibody能够检测内源性的Ser2448位点磷酸化的mTOR蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser2448 of human mTOR. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是由合成的人源的针对mTOR蛋白Ser2448位点的磷酸化肽段免疫动物而生产的。抗体由protein A和肽亲和层析技术纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells (starved for 16 hours) untreated or EGF-treated (100 ng/ml), using Phospho-mTOR (Ser2448) Antibody (upper) or control mTOR Antibody #2972 (lower).

Western blot方法检测饥饿处理16小时的293细胞提取物:未处理和EGF (100 ng/ml)处理,使用的抗体为Phospho-mTOR (Ser2448) antibody (上图) 和 对照mTOR Antibody #2972(下图)。


The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9).

哺乳动物雷帕霉素(rapamycin)靶蛋白(mTOR,FRAP,RAFT)是一种丝氨酸/苏氨酸蛋白激酶(1-3),其作为ATP和氨基酸的传感器,可以有效调控营养供应及细胞的生长(4,5)。当营养充分时,mTOR响应磷脂酸介导的信号,进而正向调节p70 S6 激酶,并参与失活eIF4E的抑制剂4E-BP1。这些信号导致特定的mRNA亚群的翻译。mTOR可以被Akt信号通路的PI3K激酶磷酸化修饰Ser2448位点,也能自我磷酸化Ser2481位点(7,8)。mTOR的在细胞生长和动态平衡中起着关键作用,在肿瘤中可能存在调控失常。因此,目前很多研究将mTOR作为一个潜在的抗肿瘤治疗靶点(9)。

  1. Sabers, C.J. et al. (1995) J Biol Chem 270, 815-22.
  2. Brown, E.J. et al. (1994) Nature 369, 756-8.
  3. Sabatini, D.M. et al. (1994) Cell 78, 35-43.
  4. Gingras, A.C. et al. (2001) Genes Dev 15, 807-26.
  5. Dennis, P.B. et al. (2001) Science 294, 1102-5.
  6. Fang, Y. et al. (2001) Science 294, 1942-5.
  7. Navé, B.T. et al. (1999) Biochem J 344 Pt 2, 427-31.
  8. Peterson, R.T. et al. (2000) J Biol Chem 275, 7416-23.
  9. Huang, S. and Houghton, P.J. (2003) Curr Opin Pharmacol 3, 371-7.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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