Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

CDK7 (MO1) Mouse mAb #2916

CAK   cyclin   MO15  

No. Size Price
2916S 100 µl ( 20 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
2916T 20 µl ( 2 western blots ) ¥1,200.00 现货查询 购买询价 防伪查询
2916 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:2000 Human,Rat, Endogenous 40 Mouse IgG2b
IP 1:200
IHC-P 1:1000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

CDK7 (MO1) Mouse mAb detects endogenous levels of total CDK7 protein. The antibody does not cross-react with other CDKs. CDK7 (MO1) Mouse mAb能够检测内源性CDK7总蛋白,该抗体不与其他CDKs发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human CDK7. 该单克隆抗体是由重组人CDK7蛋白免疫动物生产的。

Western Blotting

Western Blotting

Western blot analysis of extracts from SK-N-MC and C6 cells, using CDK7 (MO1) Mouse mAb. Western blot方法检测 SK-N-MC和C6细胞提取物,使用的抗体为 CDK7 (MO1) Mouse mAb。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using CDK7 (MO1) Mouse mAb. 免疫组织化学方法检测石蜡包埋人类乳腺癌组织,显示核定位,使用的抗体为CDK7 (MO1) Mouse mAb。


CDK-activating kinase (CAK) is a complex of CDK7 and cyclin H. The complex is involved in cell cycle regulation by phosphorylating an activating residue in the T-loop domain of cdks (1). Regulation of CAK activity is mediated by T-loop phosphorylation and by association with MAT1, both of which enhance its kinase activity toward the CTD of RNA polymerase II (2,3) and other substrates such as p53 (4). CAK is an essential component of the transcription complex TFIIH and may interact directly with TFIIH helicases (5). CDK激活激酶(CAK)是CDK7和周期蛋白H的复合体。该复合体通过磷酸化cdks的T-回路结构域的激活残基参与细胞周期的调节(1)。CAK活性的调节经由T-回路的磷酸化和MAT1的缔合来介导,这两个过程都能够增强CAK对于RNA 聚合酶II(2,3)的CTD以及其它底物如p53(4)的激酶活性。CAK是转录复合体TFIIH必不可少的组成部分,并可以直接和TFIIH解旋酶相互作用(5)。

  1. Fisher, R.P. et al. (1994) Cell 78, 713-724.
  2. Larochelle, S. et al. (2001) EMBO J. 16, 3749-3759.
  3. Yankulov, K.Y. et al. (1997) EMBO J. 16, 1638-1646.
  4. Ko, L.J. et al. (1997) Mol. Cell. Biol. 17, 7220-7229.
  5. Rossignol, M. et al. (1997) EMBO J. 16, 1628-1637.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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