Cell Signaling Technology

Product Pathways - Adhesion

Phospho-Catenin δ-1 (Tyr228) Antibody #2911

cadherin   CTNND   CTNND1   KIAA0384   p120-catenin   P120CAS   P120CTN  

No. Size Price
2911S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2911 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 95, 100 Rabbit
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Phospho-Catenin δ-1 (Tyr228) Antibody detects endogenous levels of catenin δ-1 protein only when phosphorylated at Tyr228. The antibody might cross react with another overexpressed phospho-tyrosine protein.

Phospho-Catenin δ-1 (Tyr228) 抗体仅能检测228位酪氨酸磷酸化后的内源性 catenin δ-1蛋白。该抗体可能与其他过表达的酪氨酸磷酸化蛋白发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Tyr228 of human/mouse catenin δ-1. Antibodies are purified by peptide affinity chromatography.

合成对应人/或小鼠catenin δ-1228位酪氨酸及其邻近氨基酸残基的多肽免疫动物获得多克隆抗体。抗体通过肽亲和层析纯化获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from A431 cells, serum-starved overnight and then either left untreated or treated with EGF for 15 minutes, using Phospho-Catenin δ-1 (Tyr228) Antibody (upper) and Catenin δ-1 Antibody #4989 as a loading control (lower).血清饥饿过夜随后不加处理或使用EGF处理15分钟的A431细胞提取物,使用Phospho-Catenin δ-1 (Tyr228) 抗体(上)和作为上样对照的Catenin δ-1抗体#4989(下)进行western blot分析。

IF-IC

IF-IC

Confocal immunofluorescent analysis of A431 cells, serum-starved (left) or EGF-treated (right), using Phospho-Catenin δ-1 (Tyr228) Antibody (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).血清饥饿(左)或EGF处理(右)的A431细胞,使用Phospho-Catenin δ-1 (Tyr228) 抗体(绿色)进行激光共聚焦免疫荧光分析。蓝色假色=DRAQ5™ (DNA荧光染料)。

Background

Catenin δ-1 (p120 catenin) has an amino-terminal coiled-coil domain followed by a regulatory domain containing multiple phosphorylation sites and a central Armadillo repeat domain of ten linked 42-amino acid repeats. The carboxy-terminal tail has no known function (1). Catenin δ-1 fulfills critical roles in the regulation of cell-cell adhesion as it regulates E-cadherin turnover at the cell surface to determine the level of E-cadherin available for cell-cell adhesion (2). Catenin δ-1 has both positive and negative effects on cadherin-mediated adhesion (3). Actin dynamics are also regulated by catenin δ-1, which modulates RhoA, Rac, and cdc42 proteins (1). Analogous to β-catenin, catenin δ-1 translocates to the nucleus, although its role at this location is unclear. Many studies show that catenin δ-1 is expressed irregularly or is absent in various types of tumor cells, suggesting that catenin δ-1 may function as a tumor suppressor (4).

Catenin δ-1 (p120 catenin)氨基末端是一个螺旋环-螺旋结构域,随后是带有多个磷酸化位点和一个由42个氨基酸重复10次组成的一个中心Armadillo重复结构域。羧基端功能尚不清楚(1)。Catenin δ-1调控细胞表面E-cadherin翻转以使E-cadherin达到足以形成细胞-细胞黏附,从而发挥Catenin δ-1调控细胞黏附的作用(2)。Catenin δ-1在cadherin-介导的细胞黏附过程中同时扮演正向和负向调控作用(3)。catenin δ-1同时也会调控肌动蛋白丝的动力变化过程,以调节RhoA,Rac和cdc42蛋白(1)。类似于β-catenin,catenin δ-1会转移到细胞核,尽管这种转移的目的尚不清楚。许多研究表明在多种癌细胞中,catenin δ-1表达异常或缺失,提示catenin δ-1可能作为一种抑癌因子(4)。

Catenin δ-1 is phosphorylated at multiple tyrosine sites along its sequence both in vivo and in vitro (5). High levels of catenin δ-1 phosphorylated at Tyr228 are commonly seen in several carcinoma cell lines. EGFR signaling induces catenin δ-1 phosphorylation at Tyr228, with the phosphorylated protein becoming localized at adherens junctions although phosphorylation is not essential in junction formation (6).

Catenin δ-1蛋白序列上多个酪氨酸位点可以在体内或体外被磷酸化(5)。Catenin δ-1 228位酪氨酸高水平的磷酸化在一些癌细胞系中很常见。EGFR信号会诱导Catenin δ-1 在228位酪氨酸磷酸化,并使磷酸化后的蛋白定位到黏附连接处,尽管磷酸化对细胞连接的形成并非必须(6)。

  1. Reynolds, A.B. and Roczniak-Ferguson, A. (2004) Oncogene 23, 7947-7956.
  2. Davis, M. A. et al. (2003) J. Cell Biol. 163, 525-534.
  3. Thoreson, M.A. and Reynolds, A.B. (2002) Differentiation 70, 583-589.
  4. Anastasiadis, P.Z. and Reynolds, A.B. (2000) J. Cell Sci. 113, 1319-1334.
  5. Mariner, D.J. et al. (2001) J. Biol. Chem. 276, 28006-28013.
  6. Mariner, D.J. et al. (2004) J. Cell Sci. 117, 1339-1350.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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