Cell Signaling Technology

Product Pathways - Adhesion

Phospho-Catenin δ-1 (Tyr904) Antibody #2910

cadherin   CTNND   CTNND1   KIAA0384   p120-catenin   P120CAS   P120CTN  

No. Size Price
2910S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2910 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 95, 100 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-Catenin δ-1 (Tyr904) Antibody detects endogenous levels of catenin δ-1 protein only when phosphorylated at Tyr904. The antibody might cross react with another overexpressed phospho-tyrosine protein.

Phospho-Catenin δ-1 (Tyr904) 抗体仅能识别在904位酪氨酸磷酸化后的内源性catenin δ-1蛋白。该抗体可能会与其他过表达的磷酸化酪蛋白发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Tyr904 of human/mouse catenin δ-1. Antibodies are purified by peptide affinity chromatography.

合成对应人/小鼠catenin δ-1蛋白904位酪氨酸及其邻近氨基酸残基序列的磷酸多肽免疫动物获得多克隆抗体。抗体通过肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from overnight serum starved A431 cells, untreated or EGF-treated for 15 minutes, using Phospho-Catenin δ-1 (Tyr904) Antibody (upper) and Catenin δ-1 Antibody #4989 as a loading control (lower).饥饿过夜的A431细胞随后不处理或使用EGF处理15分钟后获得细胞提取物使用Phospho-Catenin δ-1 (Tyr904) 抗体(上)进行western blot分析,Catenin δ-1 抗体#4989 作为上样对照(下)。

Background

Catenin δ-1 (p120 catenin) has an amino-terminal coiled-coil domain followed by a regulatory domain containing multiple phosphorylation sites and a central Armadillo repeat domain of ten linked 42-amino acid repeats. The carboxy-terminal tail has no known function (1). Catenin δ-1 fulfills critical roles in the regulation of cell-cell adhesion as it regulates E-cadherin turnover at the cell surface to determine the level of E-cadherin available for cell-cell adhesion (2). Catenin δ-1 has both positive and negative effects on cadherin-mediated adhesion (3). Actin dynamics are also regulated by catenin δ-1, which modulates RhoA, Rac, and cdc42 proteins (1). Analogous to β-catenin, catenin δ-1 translocates to the nucleus, although its role at this location is unclear. Many studies show that catenin δ-1 is expressed irregularly or is absent in various types of tumor cells, suggesting that catenin δ-1 may function as a tumor suppressor (4).

Catenin δ-1 (p120 catenin)氨基末端是一个螺旋环-螺旋结构域,随后是带有多个磷酸化位点和一个由42个氨基酸重复10次组成的一个中心Armadillo重复结构域。羧基端功能尚不清楚(1)。Catenin δ-1调控细胞表面E-cadherin翻转以使E-cadherin达到足以形成细胞-细胞黏附,从而发挥Catenin δ-1调控细胞黏附的作用(2)。Catenin δ-1在cadherin-介导的细胞黏附过程中同时扮演正向和负向调控作用(3)。catenin δ-1同时也会调控肌动蛋白丝的动力变化过程,以调节RhoA,Rac和cdc42蛋白(1)。类似于β-catenin,catenin δ-1会转移到细胞核,尽管这种转移的目的尚不清楚。许多研究表明在多种癌细胞中,catenin δ-1表达异常或缺失,提示catenin δ-1可能作为一种抑癌因子(4)。

Phosphorylation of Tyr904 on Catenin-δ-1 was identified at Cell Signaling Technology (CST) using PhosphoScan®, a CST's LC-MS/MS platform for phosphorylation site discovery (5).

Cell Signaling Technology (CST) 使用PhosphoScan®, 一种发现磷酸化位点的CST的液相色谱质谱平台(5)识别了Catenin-δ-1 蛋白904位酪氨酸磷酸化。

  1. Reynolds, A.B. and Roczniak-Ferguson, A. (2004) Oncogene 23, 7947-7956.
  2. Davis, M. A. et al. (2003) J. Cell Biol. 163, 525-534.
  3. Thoreson, M.A. and Reynolds, A.B. (2002) Differentiation 70, 583-589.
  4. Anastasiadis, P.Z. and Reynolds, A.B. (2000) J. Cell Sci. 113, 1319-1334.
  5. Rush, J. et al. (2005) Nat. Biotechnol. 23, 94-101.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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