Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

VEGF Receptor 2 Control Proteins #2904

extracts   flk   kdr   lysates   vegf receptor   vegf receptor 2   vegf receptor-2   vegf receptor2   vegfr   vegfr 2   vegfr-2   vegfr2  

No. Size Price
2904S 150 µl ( 10 western blots ) ¥1,413.00 现货查询 购买询价
2904 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Description

Nonphosporylated VEGFR2 Control Proteins: Purified recombinant human VEGFR2 (Val789-Val1356) expressed as a GST fusion protein in insect cells treated with λ phosphatase. Supplied in SDS Sample Buffer. Store at -20ºC. 
 Phosphorylated VEGFR2 Control Proteins: Purified recombinant human VEGFR2 (Val789-Val1356) expressed as a GST fusion protein in insect cells. Supplied in SDS Sample Buffer. Store at -20ºC.

未磷酸化的VEGFR2对照蛋白:纯化的重组的人VEGFR2 (Val789-Val1356)是经过λ磷酸酶处理的表达在昆虫细胞中的GST融合蛋白。于SDS样品缓冲液中,-20ºC保存。磷酸化的VEGFR2对照蛋白: 纯化的重组的人VEGFR2 (Val789-Val1356) 是表达在昆虫细胞中的GST融合蛋白。于SDS样品缓冲液中,-20ºC保存。

Western Blotting

Western Blotting

Western blot analysis of recombinant human GST-VEGF Receptor 2 (Val789-Val1356), untreated or λ phosphatase-treated, using Phospho-VEGF Receptor 2 (Tyr1175) Antibody #2478 (upper), VEGF Receptor 2 Antibody #2479 (lower). 用Phospho-VEGF Receptor 2 (Tyr1175) Antibody #2478 (上), VEGF Receptor 2 Antibody #2479 (下)对未处理的和经过λ phosphatase处理的重组的人的GST-VEGF Receptor 2 (Val789-Val1356)进行免疫印迹检测。

Directions for Use

As controls, we recommend using 15 µl (20 ng) of phosphorylated and nonphosphorylated VEGFR2 control proteins. Boil sample before use.

作为对照,我们推荐使用15 µl (20 ng)磷酸化的和非磷酸化的VEGFR2对照蛋白。使用前请煮样。

Background

Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, Grb2, PI-3 kinase, Nck and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). The phosphorylation of Tyr1212 provides a docking site for Grb2 binding and phospho-Tyr1175 binds with the p85 subunit of PI-3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8).

血管内皮生长因子受体2 (VEGFR2, KDR, Flk-1)是内皮细胞上VEGF诱导信号时的一个主要受体。和配体结合之后,VEGFR2发生自磷酸化并被活化(1)。VEGFR2 主要的自磷酸化受体位于激酶插入区 (Tyr951/996)和酪氨酸激酶催化区(Tyr1054/1059) (2)。受体的激活会导致对包括Shc, Grb2, PI3K激酶, Nck 和蛋白酪氨酸磷酸化酶SHP-1 和 SHP-2接头蛋白的快速招募(3)。Tyr1212的磷酸化为Grb2提供了结合位点而且磷酸化的Tyr1175能结合PI3K激酶的p85 亚基以及PLCγ和 Shb (1,4,5)。VEGFR2信号传导对于实现VEGF刺激引起的增殖、趋化和发芽,以及体外培养的内皮细胞的增殖和体内血管的发生是必需的(6-8)。

  1. Meyer, M. et al. (1999) EMBO J 18, 363-74.
  2. Dougher-Vermazen, M. et al. (1994) Biochem Biophys Res Commun 205, 728-38.
  3. Kroll, J. and Waltenberger, J. (1997) J Biol Chem 272, 32521-7.
  4. Takahashi, T. et al. (2001) EMBO J 20, 2768-78.
  5. Holmqvist, K. et al. (2004) J Biol Chem 279, 22267-75.
  6. Karkkainen, M.J. and Petrova, T.V. (2000) Oncogene 19, 5598-605.
  7. Rahimi, N. et al. (2000) J Biol Chem 275, 16986-92.
  8. Claesson-Welsh, L. (2003) Biochem Soc Trans 31, 20-4.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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