Cell Signaling Technology

Product Pathways - Protein Translation

CHOP (L63F7) Mouse mAb #2895

C/EBP   C/EBP homologous   CHOP   CHOP 10   CHOP-10   DDIT   DDIT 3   DDIT-3   DDIT3   DNA damage-inducible transcript   DNA damage-inducible transcript 3   GADD153   Growth arrest and DNA-damage-inducible protein   Growth arrest and DNA-damage-inducible protein GADD153   sc-7351  

No. Size Price
2895S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
2895T 20 µl ( 2 western blots ) ¥1,200.00 现货查询 购买询价
2895 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 27 Mouse IgG2a
IP 1:50
IF-IC 1:3200
ChIP 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP,

Specificity / Sensitivity

CHOP (L63F7) Mouse mAb detects endogenous levels of total CHOP protein.

CHOP (L63F7) Mouse mAb识别内源性的CHOP总蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human CHOP.该单克隆抗体用与人类CHOP蛋白氨基酸序列对应的人工合成肽段免疫动物而制成。

IF-IC

IF-IC

Confocal immunofluorescent analysis of A-204 cells, untreated (left) or tunicamycin-treated (right), using CHOP (L63F7) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

对A-204细胞,未处理(左)或tunicamycin处理(右)使用CHOP (L63F7) Mouse mAb(绿色)进行共聚焦免疫荧光分析。肌动蛋白用DY-554 phalloidin(红色)标记。

Western Blotting

Western Blotting

Western blot analysis of extracts from C6 and A-204 cells, untreated or treated with thapsigargin (300 nM, 2 hours) or tunicamycin (24 μg/ml, 2 hours), using CHOP (L63F7) Mouse mAb.

未处理或者加入thapsigargin (300 nM,2小时)或tunicamycin (24 ug/ml,2小时)处理C6和A204细胞,使用CHOP (L63F7) Mouse mAb进行Western blot分析。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 MEF wild-type cells treated with tunicamycin (2ug/ml) overnight, and 1 µl of CHOP (L63F7) Mouse mAb or 2 µl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse ATF-3 Intron 1 Primers #13059, mouse CHOP promoter primers, and SimpleChIP® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Background

CHOP was identified as a C/EBP-homologous protein that inhibits C/EBP and LAP in a dominant-negative manner (1). CHOP expression is induced by certain cellular stresses including starvation and the induced CHOP suppresses cell cycle progression from G1 to S phase (2). Later it was shown that, during ER stress, the level of CHOP expression is elevated and CHOP functions to mediate programmed cell death (3). Studies also found that CHOP mediates the activation of GADD34 and Ero1-Lα expression during ER stress. GADD34 in turn dephosphorylates phospho-Ser51 of eIF2α thereby stimulating protein synthesis. Ero1-Lα promotes oxidative stress inside the endoplasmic reticulum (ER) (4). The role of CHOP in the programmed cell death of ER-stressed cells is correlated with its role promoting protein synthesis and oxidative stress inside the ER (4).

CHOP被鉴定为一种C/EBP同源蛋白,以显性负调控的方式抑制C/EBP和LAP(1)。CHOP表达可以被某些细胞应激所诱导,包括饥饿,诱导的CHOP抑制细胞周期从G1进入到S期(2)。后来发现,在内质网应激时,CHOP表达水平升高,可以介导程序性细胞死亡(3)。研究也发现CHOP可以介导GADD34和Ero1-Lα在内质网应激时的表达。GADD34反过来对eIF2α的磷酸化Ser51进行去磷酸化,从而刺激蛋白合成。Ero1-Lα促进内质网内氧化应激(4)。CHOP在内质网应激后细胞程序性死亡中的角色与其能够促进内质网内蛋白合成和氧化应激有关(4)。

  1. Ron, D. and Habener, J.F. (1992) Genes Dev 6, 439-53.
  2. Barone, M.V. et al. (1994) Genes Dev 8, 453-64.
  3. Zinszner, H. et al. (1998) Genes Dev 12, 982-95.
  4. Marciniak, S.J. et al. (2004) Genes Dev 18, 3066-77.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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