Cell Signaling Technology

Product Pathways - Stem Cell and Lineage Markers

Oct-4A (C52G3) Rabbit mAb #2890

sc-25401   sc-5279   sc-8628   sc-9081  

No. Size Price
2890S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
2890 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 45 Rabbit IgG
IHC-P 1:1200
F 1:400
IF-IC 1:1600
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP,

Specificity / Sensitivity

Oct-4A (C52G3) Rabbit mAb detects endogenous levels of total Oct-4A protein.

Oct-4A (C52G3)Rabbit mAb兔单抗识别内源性的总Oct-4A 蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Oct-4A.




Confocal immunofluorescent analysis of NTERA2 cells using Oct-4A (C52G3) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

使用Oct-4A (C52G3) Rabbit mAb 兔单抗(绿色)对NTERA2细胞进行激光共聚焦荧光分析。使用DY-554 phalloidin(红色)标记肌动蛋白丝。

Western Blotting

Western Blotting

Western blot analysis of extracts from NCCIT and NTERA2 cells using Oct-4A (C52G3) Rabbit mAb.

使用Oct-4A (C52G3) Rabbit mAb兔单抗对NCCIT和NTERA2细胞提取物进行western blot分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human seminoma, showing nuclear localization, using Oct-4A (C52G3) Rabbit mAb.

使用Oct-4A (C52G3) Rabbit mAb兔单抗对石蜡包埋的人精原细胞瘤进行免疫组化分析,结果显示Oct-4A定位于细胞核。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded NTERA2 (positive, left) and HeLa (negative, right) cell pellets using Oct-4A (C52G3) Rabbit mAb.

使用Oct-4A (C52G3) Rabbit mAb兔单抗对石蜡包埋的NTERA2(阳性,左)和HeLa(阴性,右)细胞进行免疫组化分析。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells (blue) and NCCIT cells (green) using Oct-4A (C52G3) Rabbit mAb.

使用Oct-4A (C52G3) 对HeLa细胞(蓝色)和NCCIT细胞(绿色)进行流式细胞分析。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NCCIT cells and either 10 μl of Oct-4A (C52G3) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003将4 x 106 NCCIT细胞与10μl Oct-4A (C52G3)Rabbit mAb兔单抗或2μl Normal Rabbit IgG#2729进行染色质免疫共沉淀。富集到的DNA使用SimpleChIP® 人Oct-4启动子引物#4641,SimpleChIP® 人Sox2启动子引物#4649和SimpleChIP® 人α卫星重复序列引物#4486进行荧光实时PCR定量。各样品沉淀得到的DNA量显示为与input染色质总量相对定量,Input中染色质量设定值为1。


Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells (1). A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that it is the Oct-4A isoform that has the ability to confer and sustain pluripotency and that Oct-4B may exist in some somatic, non-pluripotent cells (6,7).

Oct-4 (POU5F1)是一类高表达于未分化胚胎干细胞和原始胚胎生殖细胞中的转录因子(1),一系列关键因子包括Oct-4, Nanog和 Sox2对保持多潜能性是必需的,Oct-4的下调显示能起始细胞分化(2,3)。Oct-4是一种非常有用的生殖细胞肿瘤标志物[4]。Oct-4存在两种剪接异构体Oct-4A and Oct-4B(5)。近来研究暗示Oct-4A异构体能给予并保持干细胞多潜能,而Oct-4B可能存在于某些体细胞和非多潜能性细胞中(6,7)。

  1. Looijenga, L.H. et al. (2003) Cancer Res 63, 2244-50.
  2. Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-278.
  3. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9.
  4. Cheng, L. et al. (2007) J Pathol 211, 1-9.
  5. Takeda, J. et al. (1992) Nucleic Acids Res 20, 4613-20.
  6. Cauffman, G. et al. (2006) Stem Cells 24, 2685-91.
  7. Lee, J. et al. (2006) J Biol Chem 281, 33554-65.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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