Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-c-Abl (Tyr412) (247C7) Rabbit mAb #2865

Ableson protein tyrosine kinase   Bcr-Abl   c-Abl  

No. Size Price
2865S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2865 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 135 (c-Abl); 210 (Bcr-Abl) Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Mouse,

Specificity / Sensitivity

Phospho-c-Abl (Tyr412) (247C7) Rabbit mAb detects endogenous Abl proteins only when phosphorylated at tyrosine 412. The antibody does not cross-react with other phosphorylated tyrosine kinases.

Phospho-c-Abl (Tyr412) (247C7) Rabbit mAb兔单抗能检测内源性Tyr412位点磷酸化的c-Abl蛋白水平。这种抗体不会与其他磷酸化酪氨酸激酶发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr412 of human c-Abl.

该单克隆抗体通过使用合成的与人源c-Abl蛋白Tyr412位点周围残基相一致的磷酸化肽段免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from cells expressing various activated tyrosine kinase proteins, using Phospho-c-Abl (Tyr412) (247C7 ) Rabbit mAb (upper) or Phospho-Tyrosine mAb (P-Tyr-100) #9411 (lower). Phospho-c-Abl (Tyr412) (247C7 ) Rabbit mAb specifically recognizes the activated Abl of Bcr-Abl fusion proteins, whose phosphorylation was abolished by calf intestinal phosphatase (CIP) treatment.western blot方法检测细胞提取物:表达各种有活性酪氨酸激酶蛋白的细胞,使用的抗体是Phospho-c-Abl (Tyr412) (247C7) Rabbit mAb (上图)和 Phospho-Tyrosine mAb (P-Tyr-100) #9411 (下图)。Phospho-c-Abl (Tyr412) (247C7) Rabbit mAb 能特异性识别Bcr-Abl 融合蛋白中活化的Abl蛋白,Abl蛋白的磷酸化经牛小肠磷酸酶(CIP)处理后能去磷酸化。

Western Blotting

Western Blotting

Western blot analysis of extracts from K562 cells, untreated or treated with an Abl inhibitor, using Phospho-c-Abl (Tyr412) (247C7) Rabbit mAb.western blot方法检测细胞提取物:未处理和用Abl抑制剂处理的K562细胞,使用的抗体是Phospho-c-Abl (Tyr412) (247C7) Rabbit mAb

Background

The c-Abl proto-oncogene encodes a nonreceptor protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. It is implicated in regulating cell proliferation, differentiation, apoptosis, cell adhesion and stress responses (1-3). c-Abl kinase activity is increased in vivo by diverse physiological stimuli including integrin activation, PDGF stimulation and binding to c-Jun, Nck and RFX1 (2,4). The in vivo mechanism of regulation of c-Abl kinase activity is not completely understood. Tyr245 is located in the linker region between the SH2 and catalytic domains. This positioning is conserved among Abl family members. Phosphorylation of Tyr245 is involved in the activation of c-Abl kinase (5). In addition, phosphorylation of Tyr412, which is located in the kinase activation loop of c-Abl, is required for kinase activity (6).

c-Abl原癌基因编码一个非受体蛋白酪氨酸激酶,该激酶在多细胞动物中普遍表达且高度保守。c-Abl蛋白分布于胞核以及胞质中,参与调节细胞增殖、分化、凋亡细胞粘附和应激反应(1-3)。在体内,通过整合素激活、PDGF刺激以及结合到c-Jun、Nck 和RFX1 蛋白上(2,4)等各种生理刺激,能使c-Abl激酶的活力提高。调节c-Abl蛋白激酶活力的体内机制目前尚不完全清楚。Tyr245位点位于SH2和催化区之间的连接区域,Abl蛋白家族在这个位点处都是保守的。Tyr245位点的磷酸化涉及到c-Abl激酶的活化(5)。另外,位于Abl蛋白的激酶活化环处的Tyr412位点,对激酶的活性也是必需的(6)。

  1. Wang, J.Y. et al. (2000) Oncogene 19, 5643-5650.
  2. Van Etten, R.A. et al. (1999) Trends Cell. Biol. 9, 179-182.
  3. Danial, N.N. et al. (2000) Oncogene 19, 2523-2531.
  4. Shaul, Y. et al. (2000) Cell Death Differ. 7, 10-16.
  5. Brasher, B.B. et al. (2000) J. Biol. Chem. 275, 35631-35637.
  6. Pluk, H. et al. (2002) Cell 108, 247-259.

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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