Cell Signaling Technology

Product Pathways - DNA Damage

Phospho-ATR (Ser428) Antibody #2853

ataxia telangiectasia and Rad3 related   ATM   ATM-Related   ATR   DNA-PK   FRAP-related protein 1   FRP1   PIKK Family   SCKL1  

No. Size Price
2853S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2853T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
2853 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 300 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-ATR (Ser428) Antibody detects endogenous levels of ATR only when phosphorylated at serine 428. Phospho-ATR (Ser428) Antibody 能够检测内源性的丝氨酸(428位点)磷酸化的ATR蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser428 of human ATR. Antibodies are purified by protein A and peptide affinity chromatography. 该多克隆抗体是由合成的人源的针对ATR蛋白近丝氨酸(428位)肽段免疫动物,利用A蛋白和多肽亲和层析纯化生产的。

Western Blotting

Western Blotting

Western blot analysis of Raw264.7, SV-T2 and HT-29 cells that were untreated or UV-treated (50 mJ, 30 min), using Phospho-ATR (Ser428) Antibody. Lambda phosphatase NEB #P0753 (10,000 Units/ml, 1hr) was used to demonstrate the phospho-specificity of the antibody. Western blot方法检测未处理或者紫外处理(50mJ,30min)的Raw264.7, SV-T2 和 HT-29细胞中磷酸化ATR蛋白的水平,使用的抗体为Phospho-ATR (Ser428)抗体。使用 Lambda phosphatase NEB #P0753 (10,000 Units/ml, 1hr)来展示抗体的磷酸化特异性。

Western Blotting

Western Blotting

Western blot analysis of untreated, UV-treated (50 mJ, 30 min) and nocodazole-treated (50 ng/ml, 24hr) Raw264.7 cells, using Phospho-ATR (Ser428) Antibody (upper) and a total ATR antibody (lower). Western blot方法检测未处理、紫外处理(50 mJ, 30 min)和诺考达唑处理(50 ng/ml, 24hr)Raw264.7细胞中磷酸化ATR蛋白水平(上图)和ATR总蛋白水平(下图),使用的抗体分别为 Phospho-ATR (Ser428)抗体和ATR抗体。

Background

Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are PI3 Kinase-related kinase (PIKK) family members that phosphorylate multiple substrates on serine or threonine residues that are followed by a glutamine in response to DNA damage or replication blocks (1-3). Despite the essential role of ATR in cell cycle signaling and DNA repair processes, little is known about its activation. While there have been no published reports of phosphorylation sites on ATR, Cell Signaling Technology has produced an antibody directed against phospho-ATR (Ser428) that demonstrates in vivo and UV-induced phosphorylation of this protein. This reagent could prove to be a valuable tool for monitoring ATR activation. Proline-directed phosphorylation sites like this one are often targeted by CDKs and MAPKs and can often dramatically affect protein conformation (4,5). 共济失调毛细血管扩张症突变蛋白激酶(ATM)和共济失调毛细血管扩张症兼Rad3相关激酶(ATR)属于PI3激酶相关激酶(PIKK)家族成员,它们能够在毗邻一个谷氨酰胺的丝氨酸或者苏氨酸残基处磷酸化多重底物,以响应DNA损伤或者复制阻遏(1-3)。尽管ATR在细胞周期信号和DNA修复过程中发挥重要作用,对于其激活却知之甚少。虽然ATR磷酸化位点一直没有公开的报道,CST已经生产抗体针对磷酸化的ATR(Ser428)并证明了该蛋白在体和紫外诱导的磷酸化。该试剂将是监测ATR激活的一个很有价值的工具。类似这种的脯氨酸导向的磷酸化位点通常是CDKs和MAPKs的靶标,并往往能显著影响蛋白质的构象(4,5)。

  1. Kastan, M.B. and Lim, D.S. (2000) Nat Rev Mol Cell Biol 1, 179-86.
  2. Abraham, R.T. (2004) DNA Repair (Amst) 3, 883-7.
  3. Shechter, D. et al. (2004) DNA Repair (Amst) 3, 901-8.
  4. Vauzour, D. et al. (2007) Arch Biochem Biophys 468, 159-66.
  5. Smith, J. et al. (2010) Adv Cancer Res 108, 73-112.
  6. Nam, E.A. et al. (2011) J Biol Chem 286, 28707-14.
  7. Liu, S. et al. (2011) Mol Cell 43, 192-202.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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