Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-(Ser/Thr) ATM/ATR Substrate Antibody #2851

ataxia telangiectasia mutated kinase   ATM   ATR   general   motif   PBS   phospho serine   phospho-serine   phosphoserine   rad3-related kinase   SQ   substrate   TQ  

No. Size Price
2851L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
2851S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2851 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,All Species Expected, Endogenous Rabbit
IP 1:20
E-P 1:1000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, E-P=Peptide ELISA (DELFIA),

Specificity / Sensitivity

Phospho-(Ser/Thr) ATM/ATR Substrate Antibody detects endogenous levels of proteins containing the ATM/ATR substrate motif. This antibody preferentially binds peptides and proteins that contain phospho-Ser/Thr preceded by Leu or similar hydrophobic amino acids at the -1 position and followed by Gln at the +1 position. The antibody does not cross-react with corresponding nonphosphorylated sequences or with other phospho-Ser/Thr-containing motifs. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Phospho-(Ser/Thr) ATM/ATR Substrate Antibody识别包含ATM/ATR底物基序的蛋白质。此抗体优先结合包含磷酸化丝氨酸/苏氨酸的肽段和蛋白质,还要求-1位有亮氨酸或相似的疏水氨基酸以及+1位是谷氨酰胺。此抗体不与相应的非磷酸化序列或其它包含磷酸化丝氨酸/苏氨酸的基序发生交叉反应。(美国专利号:6,441,140、6,982,318、7,259,022、7,344,714;U.S.S.N. 11,484,485;及所国外相应专利)

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic phospho-(Ser/Thr) ATM/ATR substrate peptides . Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体用合成的含磷酸化(Ser/Thr) ATM/ATR底物肽段免疫动物制备。该抗体使用蛋白A和蛋白亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, untreated or UV-treated, using Phospho-(Ser/Thr) ATM/ATR Substrate Antibody.

对未处理或UV处理的COS细胞抽提液使用Phospho-(Ser/Thr) ATM/ATR Substrate Antibody进行Western blot分析。

Western Blotting

Western Blotting

Western blot analysis of yeast cell extracts, untreated or treated with 4-NQO, a potent activator of ATM/ATR orthologues, using Phospho-(Ser/Thr) ATM/ATR Substrate Antibody.

对HeLa细胞抽提液,未处理或4-NQO(强力ATM/ATR同源物激活剂)处理,使用Phospho-(Ser/Thr) ATM/ATR Substrate Antibody进行Western blot分析。

IP

IP

Western blot analysis of immunoprecipitated protein from Chk2-transfected and UV-treated COS cells, using Chk2 antibody for immunoprecipitation and Phospho-(Ser/Thr) ATM/ATR Substrate Antibody for immunoblotting.

对转染Chk2和UV处理的COS细胞,使用Chk2 antibody进行免疫共沉淀,使用Phospho-(Ser/Thr) ATM/ATR Substrate Antibody进行免疫印迹。

ELISA-Peptide

ELISA-Peptide

Phospho-(Ser/Thr) ATM/ATR Substrate Antibody ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (S* or T* denote phosphorylated serine or threonine.)

Phospho-(Ser/Thr) ATM/ATR Substrate Antibody ELISA分析:磷酸化和非磷酸化的信噪比。(S*或T*代表磷酸化的丝氨酸或苏氨酸)

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HT-29 cells, untreated (left) or treated (right) with UV (upper) or doxorubicin (lower), using Phospho-(Ser/Thr) ATM/ATR Substrate Antibody.

对石蜡包埋的HT-29细胞,未处理(左)或UV处理(上)或多柔比星处理(下),使用Phospho-(Ser/Thr) ATM/ATR Substrate Antibody进行免疫组化分析。

Background

Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair (1). The identified substrates for ATM are p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1, and Chk1 (1,2) The essential requirement for the substrates of ATM/ATR is S*/T*Q. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate recognition by these kinases. Positively charged residues surrounding the S*/T*Q are negative determinants for substrate phosphorylation (3). The complex phenotype of AT cells suggests that it likely has additional substrates (3). To better understand the kinase and identify substrates for ATM and the related kinase ATR, CST has developed antibodies that recognize phosphorylated serine or threonine in the S*/T*Q motif.

共济失调毛细血管扩张症突变蛋白激酶(ATM)和共济失调毛细血管扩张症Rad3相关激酶(ATR)是与细胞周期检查点和DNA修复相关的激酶(1)。已发现的ATM底物包括p53、p95/NBS1、MDM2、Chk2、BRCA1、CtIP、4E-BP1和Chk1(1,2)。ATM/ATR底物的核心要求是S*/T*Q。-3和-1位的疏水氨基酸和+1位的负电荷氨基酸对这些激酶底物识别有正向决定作用。S*/T*Q附近的正电荷残基对底物磷酸化有负向决定作用(3)。AT细胞的复杂表型提示它们还有更多底物(3)。为了更好的理解激酶和鉴别ATM和ATR的相关底物,CST开发了识别S*/T*Q基序中磷酸化丝氨酸和苏氨酸的抗体。

  1. Kastan, M.B. and Lim, D.S. (2000) Nature Rev. Mol. Cell Biol. 1, 179-186.
  2. Zhao, H. and Piwnica-Worms, H. (2001) Mol. Cell. Biol. 21, 4129-4139.
  3. Kim, S. T. et al. (1999) J. Biol. Chem. 274, 37538-37543.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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