Product Pathways - Innate Immunity
IRF-9 (D8G7H) Rabbit mAb #28492
|28492S||100 µl ( 10 western blots )||￥3,100.00 现货查询||购买询价|
|28492||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation,
Specificity / Sensitivity
IRF-9 (D8G7H) Rabbit mAb recognizes endogenous levels of total IRF-9 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro362 of human IRF-9 protein.
Western blot analysis of extracts from various cell lines using IRF-9 (D8G7H) Rabbit mAb.
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Human Interferon-α1 (hIFN-α1) #8927 (10 ng/ml, 16 hr; +), using IRF-9 (D8G7H) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, IRF-9/ISGF3γ, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2).
In response to type I interferons (e.g., IFN-α, IFN-β), interferon regulatory factor 9 (IRF-9, ISGF3γ) assembles into a protein complex with Stat1 and Stat2 and translocates to the nucleus where it induces transcription of interferon response genes by binding to ISREs (3,4). Expression of IRF-9 is also induced by type I interferons (5).
- Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.
- Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.
- Fu, X.Y. et al. (1990) Proc Natl Acad Sci U S A 87, 8555-9.
- Qureshi, S.A. et al. (1995) Proc Natl Acad Sci U S A 92, 3829-33.
- Levy, D.E. et al. (1990) EMBO J 9, 1105-11.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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For Research Use Only. Not For Use In Diagnostic Procedures.
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