Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb #2842

No. Size Price
2842S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2842 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 185 Rabbit IgG
IP 1:100
IHC-P 1:1600

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin),

Homology

Species predicted to react based on 100% sequence homology: Rat,

Specificity / Sensitivity

Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb detects endogenous levels of HER3/ErbB3 proteins only when phosphorylated at Tyr1289. The antibody does not cross-react with other phosphorylated receptor tyrosine kinases at significant levels.

只有当酪氨酸(1289位)磷酸化时,磷酸化HER3/ErbB3 (Tyr1289) (D1B5) 兔单抗能够检测内源性水平的HER3/ErbB3蛋白。该抗体不与其他磷酸化受体酪氨酸激酶发生显著水平的交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1289 of human HER3/ErbB3 protein.

该单克隆抗体通过用合成磷酸肽免疫动物制备,该合成磷酸肽是人HER3/ErbB3蛋白酪氨酸(1289位)附近的残基。

Western Blotting

Western Blotting

Western blot analysis of extracts from T-47D cells (serum starved overnight), untreated (-) or treated with Human Neuregulin-1 (hNRG-1) #5218 (100 ng/ml, 15 min; +), using Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb (upper) or HER3/ErbB3 (1B2E) Rabbit mAb #4754 (lower).Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb. Western blot方法检测T-47D细胞(血清饥饿过夜)提取物,细胞未处理(-)或用人神经调节蛋白-1(hNRG-1) #5218(100 ng/ml, 15 min; +)处理,使用的抗体为Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb (上图) 或HER3/ErbB3 (1B2E) Rabbit mAb #4754 (下图).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded MCF7 cell pellets, untreated (left) or treated with hNRG-1 #5218 (right), using Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb.免疫组织化学方法检测石蜡包埋的MCF7细胞球,细胞未处理(左图)或用hNRG-1 #5218处理(右图),使用的抗体为Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HCC827 xenograft, untreated (left) or λ phosphatase-treated (right), using Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb.免疫组织化学方法检测石蜡包埋的HCC827移植物,HCC827未处理(左图)或λ-磷酸酶处理(右图),使用的抗体为Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb.免疫组织化学方法检测石蜡包埋的人肺癌组织,使用的抗体为Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded KYSE-450 cell pellets, untreated (left) or treated with Human Epidermal Growth Factor (hEGF) #8916 (right), using Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb #3777 (upper) or Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb (lower). Note lack of reactivity with Phospho-EGFR.免疫组织化学方法检测石蜡包埋的KYSE-450细胞球,细胞未处理(左图)或用人表皮生长因子(hEGF) #8916处理(右图),使用的抗体为Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb #3777 (上图) 或Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb (下图). 注意缺乏与磷酸化EGFR的反应。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HCC827 xenograft, untreated (left) or λ phosphatase-treated (right), using Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb.免疫组织化学方法检测石蜡包埋的HCC827移植物,HCC827未处理(左图)或λ-磷酸酶处理(右图),使用的抗体为Phospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb.

Background

HER3/ErbB3 is a member of the ErbB receptor protein tyrosine kinase family, but it lacks tyrosine kinase activity. Tyrosine phosphorylation of ErbB3 depends on its association with other ErbB tyrosine kinases. Upon ligand binding, heterodimers form between ErbB3 and other ErbB proteins, and ErbB3 is phosphorylated on tyrosine residues by the activated ErbB kinase (1,2). There are at least 9 potential tyrosine phosphorylation sites in the carboxy-terminal tail of ErbB3. These sites serve as consensus binding sites for signal transducing proteins, including Src family members, Grb2, and the p85 subunit of PI3 kinase, which mediate ErbB downstream signaling (3). Both Tyr1222 and Tyr1289 of ErbB3 reside within a YXXM motif and participate in signaling to PI3K (4). 
 
 Investigators have found that ErbB3 is highly expressed in many cancer cells (5) and activation of the ErbB3/PI3K pathway is correlated with malignant phenotypes of adenocarcinomas (6). Research studies have demonstrated that in tumor development, ErbB3 may function as an oncogenic unit together with other ErbB members (e.g. ErbB2 requires ErbB3 to drive breast tumor cell proliferation) (7). Thus, investigators view inhibiting interaction between ErbB3 and ErbB tyrosine kinases as a novel strategy for anti-tumor therapy.

HER3/ErbB3是ErbB酪氨酸激酶受体蛋白家族的成员之一,但却没有酪氨酸激酶活性。ErbB3的酪氨酸位点磷酸化依赖于其它ErbB酪氨酸激酶。与配体结合后,ErbB3与其它ErbB蛋白形成异二聚体,并且其酪氨酸位点能够被激活的ErbB激酶磷酸化(1,2)。在ErbB3的羧基端至少有9个潜在的酪氨酸磷酸化位点。这些位点都是信号转导蛋白一致的结合位点,这些蛋白包括Src家族的分子,Grb2, 和PI3K激酶的p85亚基,它们都能参与ErbB的下游信号调节(3)。ErbB3 的Tyr1222和Tyr1289残基都位于一个YXXM基序上并参与PI3K激酶的信号传递(4)。ErbB3高表达于很多癌细胞中(5),而ErbB3/PI3K通路的激活也和许多腺癌的恶性特征相关(6)。在肿瘤发展过程中,ErbB3可能和ErbB家族的其它成员(如,ErbB2需要和ErbB3一起促进乳腺癌细胞的增殖)作为一个原癌基因单位一起发挥功能(7)。因此,抑制ErbB3和ErbB酪氨酸激酶的相互作用已经成为抗癌治疗中的一个新策略。

  1. Yarden, Y. and Sliwkowski, M.X. (2001) Nat Rev Mol Cell Biol 2, 127-37.
  2. Guy, P.M. et al. (1994) Proc Natl Acad Sci U S A 91, 8132-6.
  3. Songyang, Z. et al. (1993) Cell 72, 767-78.
  4. Kim, H.H. et al. (1994) J Biol Chem 269, 24747-55.
  5. Sithanandam, G. et al. (2003) Carcinogenesis 24, 1581-92.
  6. Kobayashi, M. et al. (2003) Oncogene 22, 1294-301.
  7. Holbro, T. et al. (2003) Proc Natl Acad Sci U S A 100, 8933-8.

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