Cell Signaling Technology

Product Pathways - Protein Stability

LRSAM1 (D1O5S) Rabbit mAb #28405

E3 ligases   E3 Ubiquitin Ligases  

No. Size Price
28405S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
28405 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 84 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

LRSAM1 (D1O5S) Rabbit mAb recognizes endogenous levels of total LRSAM1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro143 of human LRSAM1 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using LRSAM1 (D1O5S) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human LRSAM1 protein (hLRSAM1-Myc/DDK; +), using LRSAM1 (D1O5S) Rabbit mAb.

Background

Leucine-rich repeat and sterile alpha motif-containing protein 1 (LRSAM1, hTAL1) is a multi-domain-containing E3 ubiquitin-ligase involved in the regulation of cell adhesion. The LSRAM1 protein contains amino-terminal leucine-rich repeats (LRR), an ezrin-radixin-moesin (ERM) domain, a coiled-coil region, a sterile alpha motif (SAM) domain, and a carboxy-terminal RING finger domain. Research studies demonstrate that LRSAM1 participates in the endosomal sorting of proteins by regulating the ubiquitination of Tsg101, a component of the ESCRT-I endosomal sorting complex (1). LSRAM1 ubiquitin ligase activity plays a critical role in promoting the ubiquitin-dependent autophagic clearance of pathogenic bacteria (2). Mutations in the corresponding LRSAM1 gene can contribute to a form of Charcot-Marie-Tooth (CMT2P) disease that is characterized by peripheral nervous system axonal neuropathy (3,4).

  1. Amit, I. et al. (2004) Genes Dev 18, 1737-52.
  2. Huett, A. et al. (2012) Cell Host Microbe 12, 778-90.
  3. Weterman, M.A. et al. (2012) Hum Mol Genet 21, 358-70.
  4. Nicolaou, P. et al. (2013) Eur J Hum Genet 21, 190-4.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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