Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Phospho-cPLA2 (Ser505) Antibody #2831

cPLA   cpla2   phospholipase A   PLA   PLA2  

No. Size Price
2831S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2831 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 95 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-cPLA2 (Ser505) Antibody detects endogenous levels of cPLA2 only when phosphorylated at serine 505. It does not cross-react with the secreted PLA2 (sPLA2) or cytosolic Ca2+ independent PLA2 (iPLA2).

Phospho-cPLA2(Ser505)抗体识别内源性的Ser505磷酸化的cPLA2蛋白。它不与分泌型PLA2(sPLA2)或胞浆型Ca2+非依赖PLA2(iPLA2)发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser505 of human cPLA2. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体用与人类cPLA2中Ser505位点附近的氨基酸序列对应的人工合成磷酸化的肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or TNF-α-treated (10 ng/ml for 15 minutes), and NIH/3T3 and COS cells, using Phospho-cPLA2 (Ser505) Antibody (upper) or cPLA2 Antibody #2832 (lower).

对HeLa细胞,未处理或10 ng/ml TNF-α处理15分钟,以及NIG/3T3和COS细胞,使用Phospho-cPLA2 (Ser505)抗体(上图)或cPLA2抗体#2832(下图)进行Western blot分析。

Background

Cytosolic phospholipase A2 (cPLA2) is a ubiquitously distributed enzyme that catalyzes the hydrolysis of the sn-2 acyl bond of glycerolipids to produce lysophospholipids and release arachidonic acid (1). cPLA2 has been implicated in diverse cellular responses such as mitogenesis, differentiation, inflammation and cytotoxicity (1). Calcium binding to the amino-terminal CalB domain of cPLA2 promotes the translocation of cPLA2 from cytosol to membrane, where cPLA2 cleaves arachidonic acid from natural membrane (2). Phosphorylation of cPLA2 by MAPK (p42/44 and p38) at Ser505 (3,4) and Ser727 (5) stimulates its catalytic activity.

胞浆型磷脂酶A2(cPLA2)是一个广泛分布的酶,催化甘油脂sn-2酰键水解,产生溶磷脂,释放花生四烯酸(1)。cPLA2与多种细胞反应如有丝分裂、分化、炎症和细胞毒作用等有关(1)。钙结合到cPLA2的CalB结构域氨基末端,促进cPLA2从胞质到膜的易位,并使其在膜上切割花生四烯酸(2)。MAPK(p42/44和p38)对cPLA2的Ser505(3,4)和Ser727(5)磷酸化,激活其催化活性。

  1. Leslie, C.C. (1997) J. Biol. Chem. 272, 16709-16712.
  2. Clark, J. D. et al. (1991) Cell 65, 1043-1051.
  3. Lin, L. L. et al. (1993) Cell 72, 269-278.
  4. Kramer, R.M. (1996) J. Biol. Chem. 271, 27723-27729.
  5. Hefner, Y. et al. (2000) J. Biol. Chem. 275, 37542-37551.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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