Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Phospho-cPLA2 (Ser505) Antibody #2831

cPLA   cpla2   phospholipase A   PLA   PLA2  

No. Size Price
2831S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2831 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 95 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-cPLA2 (Ser505) Antibody detects endogenous levels of cPLA2 only when phosphorylated at serine 505. It does not cross-react with the secreted PLA2 (sPLA2) or cytosolic Ca2+ independent PLA2 (iPLA2).


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser505 of human cPLA2. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or TNF-α-treated (10 ng/ml for 15 minutes), and NIH/3T3 and COS cells, using Phospho-cPLA2 (Ser505) Antibody (upper) or cPLA2 Antibody #2832 (lower).

对HeLa细胞,未处理或10 ng/ml TNF-α处理15分钟,以及NIG/3T3和COS细胞,使用Phospho-cPLA2 (Ser505)抗体(上图)或cPLA2抗体#2832(下图)进行Western blot分析。


Cytosolic phospholipase A2 (cPLA2) is a ubiquitously distributed enzyme that catalyzes the hydrolysis of the sn-2 acyl bond of glycerolipids to produce lysophospholipids and release arachidonic acid (1). cPLA2 has been implicated in diverse cellular responses such as mitogenesis, differentiation, inflammation and cytotoxicity (1). Calcium binding to the amino-terminal CalB domain of cPLA2 promotes the translocation of cPLA2 from cytosol to membrane, where cPLA2 cleaves arachidonic acid from natural membrane (2). Phosphorylation of cPLA2 by MAPK (p42/44 and p38) at Ser505 (3,4) and Ser727 (5) stimulates its catalytic activity.


  1. Leslie, C.C. (1997) J. Biol. Chem. 272, 16709-16712.
  2. Clark, J. D. et al. (1991) Cell 65, 1043-1051.
  3. Lin, L. L. et al. (1993) Cell 72, 269-278.
  4. Kramer, R.M. (1996) J. Biol. Chem. 271, 27723-27729.
  5. Hefner, Y. et al. (2000) J. Biol. Chem. 275, 37542-37551.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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