Cell Signaling Technology

Product Pathways - Apoptosis

Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb #2827

No. Size Price
2827L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
2827S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2827T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
2827 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 28 Rabbit IgG
F 1:800
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb detects endogenous levels of Bcl-2 only when phosphorylated at serine 70. The antibody does not cross-react with nonphosphorylated Bcl-2 at endogenous levels or with other Bcl-2 family members.

Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb 兔单抗只能检测内源水平的ser70位点磷酸化的Bcl-2蛋白。在内源水平上,此抗体与未磷酸化的Bcl-2和Bcl-2家族的其它成员不发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding serine 70 of human Bcl-2.

该单克隆抗体是采用合成的人 Bcl-2 蛋白 ser70 位点周围残基相对应的磷酸化肽段来免疫动物而获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated or treated with paclitaxel (1 μM, overnight) and with or without λ phosphatase, using Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb (upper) or Bcl-2 #2876 (lower).

Western blot 免疫印迹检测分别经paclitaxel (1 μM, 过夜) 处理或未处理和经λ磷酸酶处理或未处理的Jurkat细胞的细胞提取液,所用抗体是Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb 兔单抗(上图) 或者 Bcl-2 #2876 (下图) 。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, using Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb versus propidium iodide (DNA content).

流式细胞仪分析 Jurkat 细胞,所用抗体为Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb 兔单抗,与 碘化(DNA 含量)做比对。



Confocal immunofluorescent analysis of SH-SY5Y cells, untreated, using Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析SH-SY5Y 细胞(未经处理),所用抗体为Phospho-Bcl-2 (Ser70) (5H2) Rabbit mAb 兔单抗(绿色)。肌动蛋白微丝用 DY-554 phalloidin (红色)标记。Blue pseudocolor = DRAQ5® #4084 (DNA荧光染料)。


Bcl-2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release (1). It has been implicated in modulating mitochondrial calcium homeostasis and proton flux (2). Several phosphorylation sites have been identified within Bcl-2 including Thr56, Ser70, Thr74 and Ser87 (3). It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway, and that phosphorylation of Bcl-2 may be a marker for mitotic events (4,5). Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes (6). Interleukin 3 and JNK-induced Bcl-2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions (7).

Bcl-2 通过抑制线粒体细胞色素C的释放而对一系列的凋亡因素做出响应,从而发挥其促进细胞存活的功能(1)。这提示其在调节线粒体钙离子的动态平衡和质子流方面发挥着作用(2)。在Bcl-2中已经发现了几个磷酸化位点,包括Thr56, Ser70, Thr74 和 Ser87 (3)。这些磷酸化位点可能是ASK1/MKK7/JNK1通路的靶点,并且Bcl-2的磷酸化可能是有丝分裂活动的一个标志(4,5)。在T淋巴细胞糖(肾上腺)皮质激素诱导的凋亡过程中,Bcl-2在Thr56或者Ser87位点的突变能够抑制其抗凋亡的活性(6)。白细胞介素3 和JNK诱导的Bcl-2在Ser70 位点的磷酸化可能是其增强抗凋亡作用所必需的(7)。

  1. Murphy, K.M. et al. (2000) Cell Death Differ 7, 102-11.
  2. Zhu, L. et al. (1999) J Biol Chem 274, 33267-73.
  3. Maundrell, K. et al. (1997) J Biol Chem 272, 25238-42.
  4. Yamamoto, K. et al. (1999) Mol Cell Biol 19, 8469-78.
  5. Ling, Y.H. et al. (1998) J Biol Chem 273, 18984-91.
  6. Huang, S.T. and Cidlowski, J.A. (2002) FASEB J 16, 825-32.
  7. Deng, X. et al. (2001) J Biol Chem 276, 23681-8.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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