Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Phospho-PLCγ1 (Tyr783) Antibody #2821

phospholipase-C   phospholipase-C-gamma   PLC   PLC-gamma-1   plc-γ1   PLCgamma   PLCgamma1   sc-12943  

No. Size Price
2821L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
2821S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2821T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
2821 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 155 Rabbit
F 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, F=Flow Cytometry,

Specificity / Sensitivity

Phospho-PLCγ1 (Tyr783) Antibody detects PLCγ1 only when phosphorylated at tyrosine 783. It does not cross-react with phosphorylated PLCγ2 or other PLCs.

Phospho-PLCγ1 (Tyr783)抗体识别内源性的Tyr783磷酸化的PLCγ1蛋白。它不与磷酸化的PLCγ2发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding tyrosine 783 of human PLCγ1. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-stimulated for the indicated times, using Phospho-PLCγ1 (Tyr783) Antibody (upper) or PLCγ1 Antibody #2822 (lower).

对NIH/3T3细胞抽提液,未处理或PDGF刺激适当时间,使用Phospho-PLCγ1 (Tyr783)抗体(上图)或PLCγ1抗体#2822(下图)进行Western blot分析。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of NIH3T3 cells, untreated (blue) or PDGF treated (green), using Phospho-PLCγ1 (Tyr783) Antibody.

对NIH3T3细胞,未经(蓝色)或经过PDGF处理(绿色),使用Phospho-PLCγ1 (Tyr783)抗体进行流式细胞仪分析。


Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ and PLCε. The PLCβ subfamily includes four members, PLCβ1-4. All four members of the subfamily are activated by α- or β-γ-subunits of the heterotrimeric G-proteins (2,3). 
 Phosphorylation is one of the key mechanisms that regulates the activity of PLC. Phosphorylation of Ser1105 by PKA or PKC inhibits PLCβ3 activity (4,5). Ser537 of PLCβ3 is phosphorylated by CaMKII, and this phosphorylation may contribute to the basal activity of PLCβ3. PLCγ is activated by both receptor and nonreceptor tyrosine kinases (6). 
 PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783 and 1245 (7). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (8).


  1. Singer, W.D. et al. (1997) Annu Rev Biochem 66, 475-509.
  2. Smrcka, A.V. et al. (1991) Science 251, 804-7.
  3. Taylor, S.J. et al. (1991) Nature 350, 516-8.
  4. Yue, C. et al. (1998) J Biol Chem 273, 18023-7.
  5. Yue, C. et al. (2000) J Biol Chem 275, 30220-5.
  6. Margolis, B. et al. (1989) Cell 57, 1101-7.
  7. Kim, H.K. et al. (1991) Cell 65, 435-41.
  8. Wang, Z. et al. (1998) Mol Cell Biol 18, 590-7.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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