Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

Ring1A Antibody #2820


No. Size Price
2820S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
2820 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 54 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Ring1A Antibody detects endogenous levels of total Ring1A protein.

Ring1A Antibody能够检测内源性Ring1A总蛋白水平。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human Ring1A. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts of MOLT4 annd LNCaP cells using Ring1A Antibody.

使用Ring1A Antibody,免疫印迹(Western blot)分析MOLT4和LNCaP细胞中Ring1A的蛋白水平。


Ring1A plays a role in Polycomb-group protein function. Polycomb-group (PcG) proteins form two distinct complexes that are critically involved in transcriptional repression of Hox genes during development (1,2). These complexes are (a) EED-EZH2 and (b) the PRC complex, which is comprised of at least Bmi-1 and Ring1A/Ring1B. The EZH2 containing complex is responsible for the methylation of H3K27 and the PRC complex ubiquitylates H2A. EZH2 methylation is required prior to PRC ubiquitylation, and both are essential for Hox gene repression (3). It has recently been shown that PcG proteins silence a group of developmentally important regulator genes, referred to as bivalent genes (4). This regulation may be responsible for the ability of stem cells to self renew or switch to differentiate into multipotent progenitors. Aberrant epigenetic silencing by PcG proteins is also thought to be important in tumorigenesis (5).

Ring1A在Polycomb-group蛋白质功能中起着一定作用。Polycomb-group (PcG) 蛋白形成两个明显的复合物,它们在发育时期参与Hox基因的转录抑制(1,2)。这些复合物是(a) EED-EZH2和(b) the PRC复合物,这是至少由Bmi-1和Ring1A/Ring1B组成。EZH2包含的复合物对H3K27的甲基化有作用,并且PRC复合物使H2A泛素化。EZH2甲基化在PRC泛素化之前被需要,并且两者都对Hox基因抑制有本质作用(3)。最近显示PcG蛋白可沉默一群发育重要的调节基因,被称为双价基因(4)。这个调节作用对于干细胞的自我更新能力或分化进入多潜能祖细胞(multipotent progenitors,MPPs )有作用。PcG蛋白促使的异常表观基因沉默也是被认为在肿瘤的发生上有着重要作用 (5)。

  1. Cao, R. et al. (2005) Mol Cell 20, 845-54.
  2. Otte, A.P. and Kwaks, T.H. (2003) Curr Opin Genet Dev 13, 448-54.
  3. Stock, J.K. et al. (2007) Nat Cell Biol 9, 1428-35.
  4. Sparmann, A. and van Lohuizen, M. (2006) Nat Rev Cancer 6, 846-56.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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