Cell Signaling Technology

Product Pathways - Neuroscience

Complexin-1/2 (D8A6E) Rabbit mAb #28070


No. Size Price
28070S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
28070 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 14-16 Rabbit IgG
IP 1:50
IF-IC 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Complexin-1/2 (D8A6E) Rabbit mAb recognizes endogenous levels of total complexin-1 and complexin-2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro125 of human complexin-2 protein.



Confocal immunofluorescent analysis of Neuro-2a (left) and NIH/3T3 (right) cells, using Complexin-1/2 (D8A6E) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).



Immunoprecipitation of complexin-1/2 from Neuro-2A cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Complexin-1/2 (D8A6E) Rabbit mAb. Western blot analysis was performed using Complexin-1/2 (D8A6E) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full-length human complexin-1 (hCPLX-1-Myc; +) or complexin-2 (hCPLX-2-Myc; +), using Complexin-1/2 (D8A6E) Rabbit mAb (upper), and Myc-Tag (71D10) Rabbit mAb #2278 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from Neuro-2a, NIH/3T3, and mouse brain, using Complexin-1/2 (D8A6E) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).


Complexins are small soluble proteins composed of a central α-helical-structured domain surrounded by amino- and carboxy-terminal unstructured domains (1). These cytosolic proteins bind to t-SNAREs with low affinity and to assembled SNARE complexes with high affinity (1,2). Two isoforms, complexin-1 and complexin-2, are expressed in neuronal cells (3) where they regulate evoked and spontaneous exocytosis (4,5). Altered complexin expression resulting from RNAi-mediated knockdown (6) or gene invalidation (7) leads to alteration in spontaneous fusion events and neurotransmitter release, which reflects functions at both inhibitory and stimulatory synapses.

  1. Chen, X. et al. (2002) Neuron 33, 397-409.
  2. McMahon, H.T. et al. (1995) Cell 83, 111-9.
  3. Reim, K. et al. (2005) J Cell Biol 169, 669-80.
  4. Reim, K. et al. (2001) Cell 104, 71-81.
  5. Huntwork, S. and Littleton, J.T. (2007) Nat Neurosci 10, 1235-7.
  6. Maximov, A. et al. (2009) Science 323, 516-21.
  7. Xue, M. et al. (2008) Proc Natl Acad Sci U S A 105, 7875-80.

Application References

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