Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-Tyrosine Hydroxylase (Ser40) Antibody #2791

P-Tyrosine-Hydroxylase   TH   Tyrosine-3-monooxygenase   tyrosine-hydroxylase  

No. Size Price
2791S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2791 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Rat, Endogenous 55-60 Rabbit
IF-IC 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Human, Mouse,

Specificity / Sensitivity

Phospho-Tyrosine Hydroxylase (Ser40) Antibody detects endogenous levels of tyrosine hydroxylase only when phosphorylated at serine 40.

Phospho-Tyrosine Hydroxylase (Ser40) Antibody 兔多抗识别内源性的Ser40磷酸化的酪氨酸羟化酶。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence surrounding Ser40 of human tyrosine hydroxylase. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体由合成的人类酪氨酸羟化酶Ser40附近磷酸化肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。

Western Blotting

Western Blotting

Western blot analysis of extracts from PC12 cells, untreated or forskolin-treated (30 µM), using Phospho-Tyrosine Hydroxylase (Ser40) Antibody (upper) or Tyrosine Hydroxylase Antibody #2792 (lower).

Western blot分析PC12细胞(未处理,或30uM forskolin处理),使用的抗体是 Phospho-Tyrosine Hydroxylase (Ser40) Antibody 兔多抗 (上) or Tyrosine Hydroxylase Antibody 兔多抗#2792 (下) 。

IF-IC

IF-IC

Confocal immunofluorescent images of PC-12 cells, untreated (A,C) and calf intestinal phosphatase treated (B), labeled with Phospho-Tyrosine Hydroxylase (Ser40) Antibody #2791 (green, top) and Tyrosine Hydroxylase Antibody #2792 (green, bottom) compared to an isotype control (D). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析PC-12细胞,未处理(A,C)和小牛肠碱性磷酸酶处理(B),使用的抗体是 Phospho-Tyrosine Hydroxylase (Ser40) Antibody 兔多抗#2791 (绿色,顶图 ) 和Tyrosine Hydroxylase Antibody 兔多抗 #2792 (绿色,底图) ,同型对照(D)用作对照。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。

Background

Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of the neurotransmitter dopamine and other catecholamines. TH functions as a tetramer, with each subunit composed of a regulatory and catalytic domain, and exists in several different isoforms (1,2). This enzyme is required for embryonic development since TH knockout mice die before or at birth (3). Levels of transcription, translation and posttranslational modification regulate TH activity. The amino-terminal regulatory domain contains three serine residues: Ser9, Ser31 and Ser40. Phosphorylation at Ser40 by PKA positively regulates the catalytic activity of TH (4-6). Phosphorylation at Ser31 by CDK5 also increases the catalytic activity of TH through stabilization of TH protein levels (7-9).

酪氨酸羟化酶(Tyrosine hydroxylase, TH)催化神经递质多巴胺和其他儿茶酚胺类物质合成的限速步骤。TH以四聚体形式发挥功能,每个亚基都有一个调节域和一个催化域,其存在几个不同的异构体(1,2)。该酶在胚胎发育中必需,TH敲除的小鼠在出生前死亡(3)。TH的转录、翻译和翻译后修饰水平均能调节其活性。其氨基末端调节域包含三个丝氨酸残基:Ser9, Ser31和Ser40。PKA对Ser40的磷酸化正向调节TH的催化活性(4-6)。CDK5对Ser31的磷酸化也能够通过稳定TH蛋白水平来增加TH的催化活性(7-9)。

  1. Kumer, S.C. and Vrana, K.E. (1996) J Neurochem 67, 443-62.
  2. Bodeau-Péan, S. et al. (1999) J Biol Chem 274, 3469-75.
  3. Kobayashi, K. et al. (1995) J Biol Chem 270, 27235-43.
  4. Lew, J.Y. et al. (1999) Mol Pharmacol 55, 202-9.
  5. Vié, A. et al. (1999) J Biol Chem 274, 16788-95.
  6. Lindgren, N. et al. (2000) J Neurochem 74, 2470-7.
  7. Moy, L.Y. and Tsai, L.H. (2004) J Biol Chem 279, 54487-93.
  8. Lehmann, I.T. et al. (2006) J Biol Chem 281, 17644-51.
  9. Saraf, A. et al. (2007) J Biol Chem 282, 573-80.
  10. Kawahata, I. et al. (2015) Biochem Biophys Res Commun , .

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