Cell Signaling Technology

Product Pathways - Apoptosis

Phospho-FADD (Ser194) Antibody (Human Specific) #2781


No. Size Price
2781S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2781 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 28 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-FADD (Ser194) Antibody (Human Specific) detects endogenous levels of human FADD protein only when phosphorylated at serine 194.

Phospho-FADD (Ser194) Antibody (Human Specific)仅能检测内源性水平Ser194磷酸化的人源FADD蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser194 of human FADD. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的人源FADD蛋白Ser194周围残基相对应的肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat and HeLa cells, hydroxyurea-treated (4 mM, 20 hours) or nocodazole-treated (1 µg/ml, 20 hours), using Phospho-FADD Ser194 Antibody (Human Specific) (upper) or control FADD Antibody (Human Specific) #2782 (lower).

Western blot分析Jurkat和HeLa 细胞提取物,hydroxyurea(羟基脲) (4 mM, 20 hr)或(nocodazole)噻氨酯哒唑 (1 µg/ml, 20 hr)处理, 使用抗体为Phospho-FADD Ser194 Antibody (Human Specific) (上图)和control FADD Antibody (Human Specific) #2782(下图)。


Fas-associated death domain (FADD or Mort 1) functions as an important adaptor in coupling death signaling from membrane receptors, such as the Fas ligand and TNF family (DR3, DR4 and DR5), to caspase-8 (1,2). FADD has a carboxy-terminal death domain, which interacts with the cytoplasmic tail of the membrane receptor, and an amino-terminal death effector domain, which interacts with caspase-8. Clustering of the receptors upon stimulation brings about FADD and caspase-8 oligomerization, activating the caspase signaling pathway. Human FADD is phosphorylated mainly at Ser194, while mouse FADD is phosphorylated at Ser191. In both cases, the phosphorylation is cell cycle-dependent (3) and may be related to its regulatory role in embryonic development and cell cycle progression (4,5).

Fas相关死亡结构域(FADD或Mort 1)的功能是作为偶联细胞膜受体死亡信号的重要配体,如Fas配体和TNF家族(DR3,DR4和DR5),以及caspase-8(1,2)。FADD有一个与膜受体的胞质尾相互作用的羧基端的死亡结构域,和一个与caspase-8作用的氨基末端的死亡效应结构域。受体受刺激后聚集,引起FADD和caspase-8寡聚化,激活半胱天冬酶的信号转导通路。人类FADD主要在Ser194磷酸化,而小鼠FADD在Ser191磷酸化。在这两种情况下,磷酸化是细胞周期依赖性的(3),这可能与其在胚胎发育及细胞周期进程中的调节作用相关(4,5)。

  1. Ashkenazi, A. and Dixit, V.M. (1998) Science 281, 1305-1308.
  2. Kuang, A. A. et al. (2000) J. Biol. Chem. 275, 25065-25068.
  3. Scaffidi, C. et al. (2000) J. Immunol. 164, 1236-1242.
  4. Newton, K. et al. (2000) EMBO J. 19, 931-941.
  5. Zhang, J. et al. (2001) J. Biol. Chem. 276, 29815-29818.

Application References

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