Cell Signaling Technology

Product Pathways - Apoptosis

Phospho-PEA-15 (Ser104) Antibody #2776

Astrocytic phosphoprotien PEA-15   PE15   PEA15   PED   Phosphosprotein enriched in diabetes  

No. Size Price
2776L 300 µl ( 30 western blots ) ¥8,992.00 现货查询 购买询价 防伪查询
2776S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
2776 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Rat, Endogenous 15 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Mouse,

Specificity / Sensitivity

Phospho-PEA-15 (Ser104) Antibody detects endogenous levels of PEA-15 only when phosphorylated at serine 104.

Phospho-PEA-15 (Ser104) Antibody能够检测内源性的Ser104位磷酸化的PEA-15 蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser104 of human PEA-15. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的人源PEA-15蛋白Ser104周围残基想对应的磷酸化肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from serum starved human A172 and rat C6 cells, untreated or treated with 200 nM TPA, using Phospho-PEA-15 (Ser104) Antibody (upper) or PEA-15 Antibody (lower).

Western blot 检测血清饥饿处理的人A172和大鼠C6细胞的提取物,未处理 (-)和200 nM TPA处理,使用抗体为Phospho-PEA-15 (Ser104) Antibody(上图)和PEA-15 Antibody(下图)。


PEA-15 is a 15 kDa phosphoprotein expressed abundantly in astrocytes and fibroblasts as well as in tissues, including the lung and eye (1). The protein has been shown to coordinate cell growth, death, and glucose ultilization (2-4). The amino-terminal DED domain of PEA-15 mediates its binding to FADD or Erk and futher regulates the Erk and apoptosis signaling pathways. PEA-15 can be phosphorylated at two serine residues, Ser104 and Ser116, located within the carboxy terminus. Phosphorylation at these sites regulates binding to Erk and FADD (2,3).

PEA-15是一个15 kDa的磷酸化蛋白,在星形胶质细胞、成纤维细胞以及组织(包括肺和眼)中表达(1)。研究证明,该蛋白协助调控细胞的生长、死亡,及葡萄糖利用(2-4)。PEA-15的氨基末端DED结构域介导它与FADD或Erk的结合,并进一步调控Erk和细胞凋亡信号通路。PEA-15可以在羧基末端的Ser104和Ser116两个丝氨酸残基处被磷酸化。在这些位点上的磷酸化调节其与Erk和FADD的结合(2,3)。

  1. Danziger, N. et al. (1995) J. Neurochem. 64, 1016-1025.
  2. Krueger, J. et al. (2005) Mol. Biol. Cell 16, 3552-3561.
  3. Renganathan, H. et al. (2005) Biochem. J. 390, 729-735.
  4. Condorelli, G. et al. (1998) EMBO J. 17, 3858-3866.

Application References

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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