Cell Signaling Technology

Product Pathways - Adhesion

MMP-9 (D6O3H) XP® Rabbit mAb (PE Conjugate) #27647

mmp9  

No. Size Price
27647S 100 µl ( 50 tests ) ¥4,264.00 现货查询 购买询价
27647 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry,

Specificity / Sensitivity

MMP-9 (D6O3H) XP® Rabbit mAb (PE Conjugate) recognizes the full-length, proenzyme (92 kDa) and the cleaved, active enzyme (84 kDa) of MMP-9.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe542 of human MMP-9 protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated MMP-9 (D6O3H) XP® Rabbit mAb #13667.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of serum starved U-2 OS cells, untreated (blue) or treated with TPA (200 nM, 24 hr; green) using MMP-9 (D6O3H) XP® Rabbit mAb (PE Conjugate).

Background

The matrix metalloproteinases (MMPs) are a family of proteases that target many extracellular proteins including other proteases, growth factors, cell surface receptors, and adhesion molecules (1). Among the family members, MMP-2, MMP-3, MMP-7, and MMP-9 have been characterized as important factors for normal tissue remodeling during embryonic development, wound healing, tumor invasion, angiogenesis, carcinogenesis, and apoptosis (2-4). Research studies have shown that MMP activity correlates with cancer development (2). One mechanism of MMP regulation is transcriptional (5). Once synthesized, MMP exists as a latent proenzyme. Maximum MMP activity requires proteolytic cleavage to generate active MMPs by releasing the inhibitory propeptide domain from the full length protein (5).

  1. McCawley, L.J. and Matrisian, L.M. (2001) Curr Opin Cell Biol 13, 534-40.
  2. Coussens, L.M. et al. (2002) Science 295, 2387-92.
  3. Sternlicht, M.D. et al. (1999) Cell 98, 137-46.
  4. Vu, T.H. et al. (1998) Cell 93, 411-22.
  5. Nagase, H. et al. (1990) Biochemistry 29, 5783-9.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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