Cell Signaling Technology

Product Pathways - Protein Stability

ISG15 (22D2) Rabbit mAb #2758

Interferon-induced 15 kDa protein  

No. Size Price
2758S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
2758T 20 µl ( 2 western blots ) ¥1,200.00 现货查询 购买询价
2758 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 15 Rabbit IgG
IP 1:25

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

ISG15 (22D2)兔单抗识别内源性未剪切的ISG15前体总蛋白,这一抗体不识别活化(切割后)或偶联形式的ISG15,也不与其他泛素家族成员(包括泛素、SUMO1、-2、-3和NEDD8)发生交叉反应。

Source / Purification

此抗体是通过合成与人ISG15蛋白氨基末端邻近氨基酸残基序列的肽段,肽段免疫动物获得。 Western blot analysis of lysates from HeLa and A549 cells, untreated (-) or treated with IFN-α (1000 U/mL) (+) for 24 hours, using ISG15 (22D2) Rabbit mAb. 使用ISG15(22D2)兔单克隆抗体在经(+)或未经(-)α-干扰素(1000 U / ml)24小时处理的HeLa和A549细胞裂解物中检测该蛋白表达的Western blot结果。

Western Blotting

Western Blotting

Western blot analysis of lysates from HeLa and A549 cells, untreated (-) or treated with IFN-α (1000 U/mL) (+) for 24 hours, using ISG15 (22D2) Rabbit mAb.

Background

ISG15(Interferon-stimulated 15 kDa protein)是的15 kDa的干扰素刺激蛋白,也称为泛素反应性蛋白(UCRP),是泛素样蛋白家族的成员之一,并在从妊娠到天然免疫反应等多种生物学过程中发挥作用(1)。ISG15的表达由暴露于细胞的I型干扰素α和β刺激,除外病毒感染及流感B (2,3)。暴露于I型干扰素后,除外一些成纤维细胞核上皮细胞,淋巴细胞和单核细胞释放ISG15到培养基(1,4)。ISG15已经被证实作为细胞因子,通过单核细胞和巨噬细胞,刺激干扰素γ的分泌、自然杀伤细胞的增殖和中性粒细胞的趋化效应(4,5)。ISG15也被证实在细胞内发挥作用,通过E1 (Ube1L), E2 (UbcH8) 和E3连接酶通过多种类似于泛素化作用的步骤与其他蛋白共价结合(6,7)。ISG15通过泛素化加工蛋白酶Ubp43,与其它蛋白解离(8)。ISG15-蛋白结合物(ISGylation)由I型干扰素诱导,并作用于包括丝氨酸蛋白抑制Serpin 2A, PLCγ1, ERK1/2, Jak1和Stat1等靶向蛋白(9,10)。不同于泛素化作用,ISGylation并不介导蛋白的降解,相反ISGylation提高了Jak1和Stat1的活性,加强了对干扰素的细胞应答(11)。

  1. Ritchie, K.J. and Zhang, D.E. (2004) Semin. Cell Dev. Biol. 15, 237-246.
  2. Korant, B.D. et al. (1984) J. Biol. Chem. 259, 14835-14839.
  3. Haas, A.L. et al. (1987) J. Biol. Chem. 262, 11315-11323.
  4. Knight, E. and Cordova, B. (1991) J. Immunol. 146, 2280-2284.
  5. D'Cunha, J. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 211-215.
  6. Loeb, K.R. and Haas, A.L. (1992) J. Biol. Chem. 267, 7806-7813.
  7. Zhao, C. et al. (2005) Proc. Natl. Acad. Sci. USA 102, 10200-10205.
  8. Malakhov, M.P. et al. (2002) J. Biol. Chem. 277, 9976-9981.
  9. Malakhov, M.P. et al. (2003) J. Biol. Chem. 278, 16608-16613.
  10. Hamerman, J.A. et al. (2002) J. Immunol. 168, 2415-2423.
  11. Malakhova, O.A. et al. (2003) Genes Dev. 17, 455-460.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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