Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Phospho-Lck (Tyr505) Antibody #2751

Lck  

No. Size Price
2751S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2751T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
2751 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 56 Rabbit
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-Lck (Tyr505) Antibody detects endogenous levels of Lck only when phosphorylated at Tyr505. This antibody may cross-react with certain phosphorylated Src family members due to high sequence homology.

Phospho-Lck (Tyr505)抗体只能够检测内源水平在Tyr505位点磷酸化的Lck蛋白。此抗体可能与特定的磷酸化的Src家族成员交叉反应,因为高度的序列相似。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr505 of human Lck. Antibodies are purified by protein A and peptide affinity chromatography.

此多克隆抗体是通过合成人源对应的Lck Tyr505位点周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells (starved for 16 hours) treated with calf intestinal alkaline phosphatase (CIP) or H2O2 (2 mM), using Phospho-Lck (Tyr505) Antibody (upper) or control Lck Antibody #2752 (lower).Western免疫印迹。用Phospho-Lck (Tyr505) Antibody (上图) 或 control Lck Antibody #2752 (下图) 研究经牛肠道碱性磷酸酶(CIP)处理或H2O2 (2 mM)处理的饥饿16小时Jurkat细胞的细胞提取液。

Background

The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).

Src酪氨酸激酶蛋白家族包括Src, Lyn, Fyn, Yes, Lck, Blk 和 Hck, 其对真核细胞的生长和分化过程中起了重要的作用(1)。Src的活性是通过两个位点酪氨酸的磷酸化进行调节的, 但是具有相反地作用。在激酶活性环内的Tyr416 位点的磷酸化增加酶的活性,通过Csk磷酸化C-末端的Tyr527位点降低酶的活性(2)。

Lck对T淋巴细胞的激活和分化有着非常重要的作用(3,4),C-末端Tyr505位点的磷酸化降低Lck的催化活性, 同时Tyr394位点的磷酸化导致了Lck活性的增加(5)。

  1. Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609.
  2. Hunter, T. (1987) Cell 49, 1-4.
  3. Molina, T. J. et al. (1992) Nature 357, 161-164.
  4. Straus, D. B. et al. (1992) Cell 70, 585-593.
  5. Chow, L. M. et al. (1993) Nature 365, 156-160.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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