Cell Signaling Technology

Product Pathways - Development

Oct-4 Antibody #2750

Oct   OCT-4   OCT4   sc-25401   sc-5279   sc-8628   sc-9081   Sox  

No. Size Price
2750S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
2750 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 45 Rabbit
IHC-P 1:200
F 1:200
IF-IC 1:400
ChIP 1:25

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP,


Species predicted to react based on 100% sequence homology: Monkey,

Specificity / Sensitivity

Oct-4 Antibody detects endogenous levels of total Oct-4 protein.

Oct-4抗体识别内源性的总Oct-4 蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids near the carboxy terminus of human Oct-4. Antibodies are purified by peptide affinity chromatography.


Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated NCCIT cells using Oct-4 Antibody (blue) compared to a nonspecific negative control antibody (red).


Western Blotting

Western Blotting

Western blot analysis of NCCIT and NTERA2 cell extracts using Oct-4 Antibody.

使用 Oct-4抗体对NCCIT和NTERA2细胞提取物进行western blot分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human seminoma (left) or normal testis (right), showing nuclear localization using Oct-4 Antibody.




Confocal immunofluorescent analysis of NCCIT cells using Oct-4 Antibody (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red).

使用 Oct-4抗体(绿色)和 β-Tubulin (9F3) Rabbit mAb兔单抗(Alexa Fluor® 555 偶联)#2116(红色)对NCCIT细胞进行激光共聚焦荧光分析。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NCCIT cells and either 20 μl of Oct-4 Antibody #2750 or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

使用SimpleChIP® Enzymatic Chromatin IP Kit (磁珠偶联) #9003将4 x 106 NCCIT细胞与20μl Oct-4抗体或2μl Normal Rabbit IgG#2729进行染色质免疫共沉淀。富集到的DNA使用SimpleChIP® 人Oct-4启动子引物#4641,SimpleChIP® 人Sox2启动子引物#4649和SimpleChIP® 人α卫星重复序列引物#4486进行荧光实时PCR定量。各样品沉淀得到的DNA量出示相对于input染色质总量进行相对定量,Input染色质量设定值为1。


Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells (1). A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that it is the Oct-4A isoform that has the ability to confer and sustain pluripotency and that Oct-4B may exist in some somatic, non-pluripotent cells (6,7).

Oct-4 (POU5F1)是一类高表达于未分化胚胎干细胞和原始胚胎生殖细胞中的转录因子(1),一系列关键因子包括Oct-4, Nanog和 Sox2对保持多潜能性是必需的,Oct-4的下调显示能起始细胞分化(2,3)。Oct-4是一种非常有用的生殖细胞肿瘤标志物[4]。Oct-4存在两种剪接异构体Oct-4A and Oct-4B(5)。近来研究暗示Oct-4A异构体能给予并保持干细胞多潜能,而Oct-4B可能存在于某些体细胞和非多潜能性细胞中(6,7)。

  1. Looijenga, L.H. et al. (2003) Cancer Res 63, 2244-50.
  2. Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-278.
  3. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9.
  4. Cheng, L. et al. (2007) J Pathol 211, 1-9.
  5. Takeda, J. et al. (1992) Nucleic Acids Res 20, 4613-20.
  6. Cauffman, G. et al. (2006) Stem Cells 24, 2685-91.
  7. Lee, J. et al. (2006) J Biol Chem 281, 33554-65.

Application References

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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