Cell Signaling Technology

Product Pathways - Development

Sox2 Antibody #2748

Oct   SOX   SOX-2  

No. Size Price
2748S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
2748 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 35 Rabbit
IP 1:100
ChIP 1:25

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP,

Homology

Species predicted to react based on 100% sequence homology: Rat, Monkey, Bovine, Dog, Horse,

Specificity / Sensitivity

Sox2 Antibody detects endogenous levels of total Sox2 protein.

Sox2抗体识别内源性的总Sox2蛋白.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Gly179 of human Sox2. Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体是通过一种合成的肽段去免疫动物产生。这种合成的肽段与邻近人源Sox2蛋白Gly179的氨基酸残基序列一致。抗体由蛋白A和肽段亲和层析技术纯化.

Western Blotting

Western Blotting

Western blot analysis of extracts from NCCIT and NTERA2 cells using Sox2 Antibody.使用Sox2抗体对NCCIT和NTERA2细胞提取物进行western blot分析。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 NCCIT cells and either 20 μl of Sox2 Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Oct-4 Promoter Primers #4641, SimpleChIP® Human Sox2 Promoter Primers #4649, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003将4 x 106 NCCIT细胞与20μl Sox2抗体或2μl正常兔IgG#2729进行染色质免疫共沉淀。富集到的DNA使用SimpleChIP®人Oct-4启动子引物#4641,SimpleChIP®人Sox2启动子引物#4649和SimpleChIP®人α卫星重复序列引物#4486进行荧光实时PCR定量。各样品沉淀得到的DNA量通过与input染色质总量对比获得。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 mouse embryonic stem cells and either 20 μl of Sox2 Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Mouse Oct-4 Promoter Primers #4653, SimpleChIP® Mouse XIST Intron 1 Primers #4659, and the negative control SimpleChIP® Mouse RPL30 Intron 2 Primers #7015. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003将4 x 106小鼠胚胎干细胞与20μl Sox2抗体或2μl正常兔IgG#2729进行染色质免疫共沉淀。富集到的DNA使用SimpleChIP®鼠Oct-4启动子引物#4653,SimpleChIP®小鼠XIST内含子1引物#4659,和阴性对照SimpleChIP®小鼠RPL30内含子2引物#7015进行荧光实时PCR定量。各样品沉淀得到的DNA量通过与input染色质总量对比获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from NCCIT, mouse embryonic stem cells (mESCs), and F9 cells using Sox2 Antibody.使用Sox2对NCCIT,小鼠胚胎干细胞(mESCs)和F9细胞提取物进行western blot分析。

Background

Embryonic stem cells are derived from the inner cell mass of the blastocyst and are unique in their pluripotent capacity and potential for self-renewal. Sox2 is one of a set of transcription factors that are crucial for the maintenance of pluripotency (1). Sox2, Oct-4, and Nanog cooperate in this network (1-3) and siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency (4,5). Chromatin immunoprecipitation experiments have shown that Sox2 and Oct-4 bind to thousands of gene regulatory sites, highlighting the importance of these transcription factors in early embryonic development (6,7). It has recently been shown that Sox2 is amplified in lung and esophageal squamous cell tumors (8).

胚胎干细胞分离自囊泡的内细胞团,并有独特的多分化潜能和自我更新潜能。Sox2是一种对于保持细胞多潜能性起关键作用的转录因子(1)。Sox2, Oct-4和Nanog在调控网络中协同作用(1-3)。表达Sox2 或 Oct-4的siRNA诱导沉默会导致细胞多潜能性的丢失(4,5)。染色质免疫沉淀实验显示Sox2和 Oct-4能结合到数千个基因的调控位点,显示了这些转录因子在胚胎早期发育中的重要性(6,7)。最近的研究发现Sox2基因在肺癌和食管鳞癌中扩增(8).

  1. Conley, B.J. et al. (2004) Int J Biochem Cell Biol 36, 555-67.
  2. Pesce, M. and Schöler, H.R. (2001) Stem Cells 19, 271-8.
  3. Pan, G. and Thomson, J.A. (2007) Cell Res 17, 42-9.
  4. Boyer, L.A. et al. (2005) Cell 122, 947-56.
  5. Loh, Y.H. et al. (2006) Nat Genet 38, 431-40.
  6. Matin, M.M. et al. (2004) Stem Cells 22, 659-68.
  7. Takahashi, K. and Yamanaka, S. (2006) Cell 126, 663-76.
  8. Okita, K. et al. (2007) Nature 448, 313-7.
  9. Arnold, K. et al. (2011) Cell Stem Cell 9, 317-29.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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