Cell Signaling Technology

Product Pathways - Protein Stability

ISG15 Antibody #2743

Interferon-induced 15 kDa protein  

No. Size Price
2743S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
2743 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Monkey, Endogenous 15 Rabbit
F 1:200
E-P 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, F=Flow Cytometry, E-P=Peptide ELISA (DELFIA),

Specificity / Sensitivity

此抗体识别游离和偶联形式的ISG15,但不与其他泛素家族成员(包括泛素、SUMO1、-2、-3和NEDD8)发生交叉反应。

Source / Purification

此抗体是通过合成与人ISG15蛋白氨基末端氨基酸序列一致的肽段,免疫动物获得,抗体经Protein A和肽亲和层析纯化。 Western blot analysis of lysates from HeLa, A549, RAW and COS cells, treated with or without IFN (1000 U/mL) for 24 hours, using ISG15 antibody. 使用ISG15抗体在经(+)或未经(-)α-干扰素(1000 U / ml)24小时处理的HeLa、A549、RAW和COS等细胞裂解物中检测该蛋白表达的Western blot结果。 Western blot analysis of NEDD8, Ubiquitin, ISG15 and SUMO-2/3 recombinant proteins (5 ng each), using NEDD8 (#2745) , Ubiquitin (#3936), ISG15 (#2743) and SUMO-2/3 (#4974) Antibodies. 分别使用NEDD8 (#2745)、Ubiquitin (#3936)、ISG15 (#2743)和SUMO-2/3 (#4974)抗体对各5ng的NEDD8、泛素、ISG15和SUMO-2/3重组蛋白进行Western blot检测的结果。 Flow cytometric analysis of Hela cells, untreated (blue) or IFNa treated (green), using ISG15 antibody compared to a nonspecific negative control antibody (red). 使用ISG15抗体对未经IFNa处理(蓝色)或经IFNa处理(绿色)的Hela细胞进行流式细胞分析的结果,非特异性阴性抗体检测结果作为对照(红色)。 The relationship between recombinant ISG15 protein concentration and assay optical density readings. Recombinant NEDD8 protein was used as a negative control. 重组ISG15蛋白的浓度和测定的光密度读数之间的关系,重组NEDD8蛋白被用来作为阴性对照。

Western Blotting

Western Blotting

Western blot analysis of lysates from HeLa, A549, RAW and COS cells, treated with or without IFN (1000 U/mL) for 24 hours, using ISG15 antibody.

Western Blotting

Western Blotting

Western blot analysis of NEDD8, Ubiquitin, ISG15 and SUMO-2/3 recombinant proteins (5 ng each), using NEDD8 (#2745) , Ubiquitin (#3936), ISG15 (#2743) and SUMO-2/3 (#4974) Antibodies.

ELISA-Peptide

ELISA-Peptide

The relationship between recombinant ISG15 protein concentration and assay optical density readings. Recombinant NEDD8 protein was used as a negative control.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Hela cells, untreated (blue) or IFNa treated (green), using ISG15 antibody compared to a nonspecific negative control antibody (red).

Background

ISG15(Interferon-stimulated 15 kDa protein)是的15 kDa的干扰素刺激蛋白,也称为泛素反应性蛋白(UCRP),是泛素样蛋白家族的成员之一,并在从妊娠到天然免疫反应等多种生物学过程中发挥作用(1)。ISG15的表达由暴露于细胞的I型干扰素α和β刺激,除外病毒感染及流感B (2,3)。暴露于I型干扰素后,除外一些成纤维细胞核上皮细胞,淋巴细胞和单核细胞释放ISG15到培养基(1,4)。ISG15已经被证实作为细胞因子,通过单核细胞和巨噬细胞,刺激干扰素γ的分泌、自然杀伤细胞的增殖和中性粒细胞的趋化效应(4,5)。ISG15也被证实在细胞内发挥作用,通过E1 (Ube1L), E2 (UbcH8) 和E3连接酶通过多种类似于泛素化作用的步骤与其他蛋白共价结合(6,7)。ISG15通过泛素化加工蛋白酶Ubp43,与其它蛋白解离(8)。ISG15-蛋白结合物(ISGylation)由I型干扰素诱导,并作用于包括丝氨酸蛋白抑制Serpin 2A, PLCγ1, ERK1/2, Jak1和Stat1等靶向蛋白(9,10)。不同于泛素化作用,ISGylation并不介导蛋白的降解,相反ISGylation提高了Jak1和Stat1的活性,加强了对干扰素的细胞应答(11)。

  1. Ritchie, K.J. and Zhang, D.E. (2004) Semin. Cell Dev. Biol. 15, 237-246.
  2. Korant, B.D. et al. (1984) J. Biol. Chem. 259, 14835-14839.
  3. Haas, A.L. et al. (1987) J. Biol. Chem. 262, 11315-11323.
  4. Knight, E. and Cordova, B. (1991) J. Immunol. 146, 2280-2284.
  5. D'Cunha, J. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 211-215.
  6. Loeb, K.R. and Haas, A.L. (1992) J. Biol. Chem. 267, 7806-7813.
  7. Zhao, C. et al. (2005) Proc. Natl. Acad. Sci. USA 102, 10200-10205.
  8. Malakhov, M.P. et al. (2002) J. Biol. Chem. 277, 9976-9981.
  9. Malakhov, M.P. et al. (2003) J. Biol. Chem. 278, 16608-16613.
  10. Hamerman, J.A. et al. (2002) J. Immunol. 168, 2415-2423.
  11. Malakhova, O.A. et al. (2003) Genes Dev. 17, 455-460.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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