Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Phospho-MARCKS (Ser152/156) Antibody #2741

MARCKS   P-MARCKS  

No. Size Price
2741S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
2741 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 75 mouse, rat. 80 human. Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-MARCKS (Ser152/156) Antibody detects endogenous levels of MARCKS only when phosphorylated at serines 152 and 156.

Phospho-MARCKS(Ser152/156)抗体识别内源性的Ser152和Ser156磷酸化的MARCKS蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser152/156 of rat MARCKS. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体用与大鼠MARCKS中Ser152/156位点附近的氨基酸序列对应的人工合成磷酸化的肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 and C6 cells starved for 16 hours and treated with TPA (0.2 µM) for 30 minutes.

对NIH/3T3和C6细胞饥饿处理16小时后经0.2uM TPA处理30分钟,抽提液进行Western blot分析。

Background

Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) is a major PKC substrate expressed in many cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic, and mitogenesis (1). PKC phosphorylates Ser159, 163, 167, and 170 of MARCKS in response to growth factors and oxidative stress. Phosphorylation at these sites regulates the calcium/calmodulin binding and filamentous (F)-actin cross-linking activities of MARCKS (2-4). Phosphorylation by PKC also results in translocation of MARCKS from the plasma membrane to the cytoplasm (5).

豆寇酰化富含丙氨酸C-激酶底物(MARCKS)是一种重要的PKC底物,在多种细胞类型中表达。 MARCKS与细胞活力,细胞粘附,吞噬,膜运输和有丝分裂有关(1)。PKC响应生长因子和氧化应激磷酸化MARCKS的丝氨酸(159,163,167和170位),调节钙/钙调蛋白结合和MARCKS丝状(F)-肌动蛋白交联反应活性(2-4)。PKC磷酸化作用导致MARCKS从细胞膜到细胞质迁移(5)。

  1. Ramsden, J.J. (2000) Int J Biochem Cell Biol 32, 475-9.
  2. Heemskerk, F.M. et al. (1993) Biochem Biophys Res Commun 190, 236-41.
  3. Graff, J.M. et al. (1989) J Biol Chem 264, 21818-23.
  4. Hartwig, J.H. et al. (1992) Nature 356, 618-22.
  5. Thelen, M. et al. (1991) Nature 351, 320-2.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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