Cell Signaling Technology

Product Pathways - Neuroscience

Rhodopsin (D4B9B) Rabbit mAb #27182

sc-57432  

No. Size Price
27182S 100 µl ( 400 tests ) ¥3,100.00 现货查询 购买询价
27182 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
IHC-P 1:1000 Human,Mouse,Rat, Endogenous 37 Rabbit IgG
IF-F 1:400

Species cross-reactivity is determined by western blot.

Applications Key: IHC-P=Immunohistochemistry (Paraffin), IF-F=Immunofluorescence (Frozen),

Specificity / Sensitivity

Rhodopsin (D4B9B) Rabbit mAb recognizes endogenous levels of total rhodopsin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu194 of human rhodopsin protein.

IF-F

IF-F

Confocal immunofluorescent analysis of rat retina using Rhodopsin (D4B9B) Rabbit mAb (green) and GFAP (GA5) Mouse mAb #3670 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse retina using Rhodopsin (D4B9B) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded rat retina using Rhodopsin (D4B9B) Rabbit mAb.

Background

Rhodopsin is the photoreceptor in the retinal rods. It is activated by photons, transduces visual information through its cognate G protein, transducin, and is inactivated by arrestin binding (1). Using atomic-force microscopy, rhodopsin was found to be arranged into paracrystalline arrays of dimers in mouse disc membranes (2). Rhodopsin is considered to be the prototype of G protein-coupled receptors (GPCRs), and is the first GPCR for which a crystal structure was solved (3). Research studies have linked mutations in the gene encoding rhodopsin to retinitis pigmentosa (4,5), a disease characterized by retinal degeneration resulting in reduced peripheral vision and night blindness (6).

  1. Arshavsky, V.Y. and Burns, M.E. (2012) J Biol Chem 287, 1620-6.
  2. Fotiadis, D. et al. (2003) Nature 421, 127-8.
  3. Palczewski, K. et al. (2000) Science 289, 739-45.
  4. Rivolta, C. et al. (2002) Hum Mol Genet 11, 1219-27.
  5. Wilson, J.H. and Wensel, T.G. (2003) Mol Neurobiol 28, 149-58.
  6. Hartong, D.T. et al. (2006) Lancet 368, 1795-809.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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