Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

Histone H2A.Z Antibody #2718

H2A.Z   H2A/z   H2AZ  

No. Size Price
2718S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
2718 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,Zebrafish, Endogenous 14 Rabbit
IP 1:25
IF-IC 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Chicken, Xenopus, Bovine,

Specificity / Sensitivity

Histone H2A.Z Antibody detects endogenous levels of histone H2A.Z protein. The antibody does not cross-react with other histone proteins, including histone H2A.

Histone H2A.Z Antibody能够检测内源性histone H2A.Z蛋白水平。该抗体不能与其它组蛋白发生交叉反应,包括histone H2A。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human histone H2A.Z. Antibodies are purified by protein A and peptide affinity chromatography.

通过人工合成人源histone H2A.Z蛋白羧基端相应的多肽片段去免疫动物从而制备此多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, using Histone H2A.Z Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).

使用Histone H2A.Z Antibody (绿色)标记,共聚焦免疫荧光分析HeLa细胞。Alexa Fluor® 555 phalloidin标记微丝蛋白(红色)。

Western Blotting

Western Blotting

Western blot analysis of HeLa, NIH/3T3, H-4-II-E and COS cell lysates, in addition to 10 ng of recombinant H2A (rH2A) and H2A.Z (rH2A.Z) protein, using Histone H2A.Z Antibody #2718 (upper) and Histone H2A Antibody II #2578 (lower).

使用Histone H2A.Z Antibody #2718 (上图)和Histone H2A Antibody II #2578 (下图),免疫印迹(Western blot)分析HeLa、NIH/3T3、H-4-II-E和COS细胞中Histone H2A.Z和Histone H2A的蛋白水平。

Background

Modulation of chromatin structure plays a critical role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. In addition to the growing number of post-translational histone modifications regulating chromatin structure, cells can also exchange canonical histones with variant histones that can directly or indirectly modulate chromatin structure (1). There are five major variants of histone H2A: canonical H2A (most abundant), H2A.X, MacroH2A, H2ABbd and H2A.Z (2). Histone H2A.Z, the most conserved variant across species, functions as both a positive and negative regulator of transcription and is important for chromosome stability (2). Several homologous protein complexes, such as SWR-C ( S. cerivisiae), TIP60 (D. melanogaster) and SRCAP (mammals), have been shown to catalyze the ATP-dependent exchange of H2A.Z for H2A in the nucleosome (3,4,5). This exchange of histone H2A variants changes histone-histone interactions in the nucleosome core and alters an acidic patch on the surface of the nucleosome, resulting in changes in nucleosome stability and binding of non-histone proteins such as HP1α (6,7).

染色质结构的修饰在调节真核细胞的转录中扮演着重要的角色。由DNA围绕和四个中心组蛋白(H2A,H2B,H3和H4)共同组成的核小体是染色质的主要组成。除了越来越多的翻译后组蛋白修饰调节染色质的结构之外,细胞也能够将常规组蛋白交换成组蛋白变体,这能够直接或间接调节染色质结构(1)。histone H2A有五个主要变体:canonical H2A (最丰富的)、H2A.X、MacroH2A、H2ABbd和H2A.Z (2)。在整个物种中最保守的Histone H2A.Z的功能作为一个阳性和阴性的转录调节因子和在染色质稳定性中起着重要作用(2)。数种同源蛋白质复合物例如SWR-C ( S. cerivisiae), TIP60 (D. melanogaster) and SRCAP (mammals),研究显示它们在核小体中可催化ATP依赖的H2A.Z交换成H2A(3,4,5)。histone H2A变体的交换可改变在核小体中心中组蛋白-组蛋白之间的相互作用,并且改变核小体的表面的酸性结构,这导致核小体稳定性和非组蛋白例如HP1α的结合的改变(6,7)。

  1. Jin, J. et al. (2005) Trends Biochem Sci 30, 680-7.
  2. Raisner, R.M. and Madhani, H.D. (2006) Curr Opin Genet Dev 16, 119-24.
  3. Mizuguchi, G. et al. (2004) Science 303, 343-8.
  4. Kusch, T. et al. (2004) Science 306, 2084-7.
  5. Ruhl, D.D. et al. (2006) Biochemistry 45, 5671-7.
  6. Suto, R.K. et al. (2000) Nat Struct Biol 7, 1121-4.
  7. Fan, J.Y. et al. (2004) Mol Cell 16, 655-61.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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